半岛bd体育手机客户端 说明
一般描述
A highly potent, cell-permeable, selective inhibitor of Rho-associated protein kinase (Ki= 140 nM for p160ROCK(ROCK-I). Also inhibits ROCK-II with almost equal potency. Inhibition is achieved by competing with ATP for binding to the catalytic site. Exhibits 10- to 50-fold lower affinity for PKCεthan p160ROCK. Does not affect the activity of p21-activated protein kinase (PAK) even at high concentrations (~100 mM). Also acts as a potent inhibitor of agonist-induced Ca2+sensitization of myosin phosphorylation and smooth muscle contractions (IC50= 700 nM for phenylephrine-induced contractions in aortic strips).
A 5 mM (500 ?g/296 ?l) aqueous solution of Y-27632 (Cat. No. 688000). A highly potent, cell-permeable, selective inhibitor of Rho-associated protein kinase (Ki= 140 nM for p160ROCK(ROCK-I). Also inhibits ROCK-II with almost equal potency. Inhibition is achieved by competing with ATP for binding to the catalytic site. Exhibits 10- to 50-fold lower affinity for PKCεthan p160ROCK. Does not affect the activity of p21-activated protein kinase (PAK) even at high concentrations (~100 mM). Also acts as a potent inhibitor of agonist-induced Ca2+sensitization of myosin phosphorylation and smooth muscle contractions (IC50= 700 nM for phenylephrine-induced contractions in aortic strips).
包装
500 μg in Plastic ampoule
Packaged under inert gas
生化/生理作用
Target IC50: 700 nM against phenylephrine-induced contractions in aortic strips
Cell permeable: yes
Target Ki: 140 nM for p160ROCK (ROCK-I)
Reversible: no
Product competes with ATP.
Primary Target
p160ROCK
警告
Toxicity: Harmful (C)
外形
A 5 mM (500 ?g/296 ?l) solution of Y-27632 (Cat. No. 688000) in H?O.
重悬
Following initial thaw, aliquot and freeze (-20°C).
其他说明
Chitaley, K., et al. 2001.Nat. Med.7,119.
Davies, S.P., et al. 2000.Biochem. J.351,95.
Narumiya, S., et al. 2000.Methods Enzymol.325,273.
Hirose, M., et al. 1999.J. Cell Biol.141,1625.
Maekawa, M., et al. 1999.Science285,895.
Uehata, M., et al. 1997.Nature389,990.
法律信息
Sold under license of PCT Application W098/06,433A1 and under license from Mitsubishi Pharma Tanabe Corporation.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
基本信息
| 经验(实验)分子式 | C14H21N3O · 2HCl · xH2O |
| 分子量 | 320.26 (anhydrous basis) |
半岛bd体育手机客户端 性质
| 质量水平 | 100 |
| 测定 | ≥95% (HPLC) |
| 形式 | liquid |
| manufacturer/tradename | Calbiochem? |
| 储存条件 | OK to freeze protect from light |
| 运输 | wet ice |
| 储存温度 | ?20℃ |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 2 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
