Histone Deacetylase 8, His·Tag^?, Human Recombinant,S. frugiperda

半岛bd体育手机客户端 说明

一般描述

Full length, recombinant, human histone deacetylase 8 (HDAC8) fused to at the C-terminus to a His?Tag sequence at and expressed inS. frugiperdainsect cells using a baculovirus expression system. HDAC8 phosphorylatedin vivoby PKA results in decreased activity, which leads to the hyperacetylation of histones H3 and H4. HDAC8 may play a role in one of the most frequent types of acute myeloid leukemia (AML). The active deacetylase is useful in the study of HDAC8 regulation and for inhibitor screening.

包装

50 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

警告

Toxicity: Standard Handling (A)

单位定义

One unit is defined as the the amount of enzyme required to deacetylate 1 picomol fluorescent peptide substrate [Arg-His-Lys(Ac)-Lys(Ac)] per min at 30°C.

外形

In 138 mM NaCl, 25 mM Tris-HCl, 10% glycerol, 0.05% TWEEN^?-20 detergent, pH 8.0.

重悬

Following initial thaw, aliquot and freeze (-70°C).

其他说明

Waltregny, D., et al. 2005.FASEB J.19,966.
Buggy, J.J., et al. 2000.Biochem J.350,199

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
TWEEN is a registered trademark of Croda International PLC

半岛bd体育手机客户端 性质

重组	expressed in insect cells (S. frugiperda)
质量水平	100
测定	≥90% (SDS-PAGE)
形式	liquid
specific activity	≥10 units/μg protein
manufacturer/tradename	Calbiochem?
储存条件	OK to freeze avoid repeated freeze/thaw cycles
运输	wet ice
储存温度	?70℃

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 1