FPTase, GST-Fusion, His·Tag^?, Rat, Recombinant,E. coli

半岛bd体育手机客户端 说明

一般描述

Recombinant, rat FPTase composed of an α- and a β-subunit (74 and 46 kDa, respectively). The α-subunit is expressed as a GST-fusion construct with an N-terminal His?Tag sequence. Both the GST moiety and the His?Tag sequence can be utilized to facilitate enzyme capturing in prenylation and binding studies or, when desired, can also be cleaved off by TEV protease. Requires Zn²⁺and Mg²⁺for optimal activity.
Recombinant, rat FPTase composed of an α- and a β-subunit (74 and 46 kDa, respectively). The α-subunit is expressed as a GST-fusion protein with an N-terminal His?Tag sequence. Both the GST moiety and the His?Tag sequence can be utilized to facilitate enzyme capturing in prenylation and binding studies or, when desired, can also be cleaved off by TEV protease. Requires Zn²⁺and Mg²⁺for optimal activity.

包装

100 μg in Plastic ampoule

生化/生理作用

1 pmol FTase will transfer ≥1 pmol farnesyl to H-Ras in 10 min at 37°C, pH 7.2.

警告

Toxicity: Standard Handling (A)

外形

In 40 mM NaCl, 25 mM HEPES, 5 mM DTT, pH 7.2.

重悬

Following initial thaw, aliquot and freeze (-70°C).

其他说明

Zimmerman, K.K., et al. 1998.Protein Expr. Purif.14,395.
Reiss, Y., et al. 1990.Cell62,81.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

半岛bd体育手机客户端 性质

质量水平	100
重组	expressed inE. coli
测定	≥90% (SDS-PAGE)
形式	liquid
manufacturer/tradename	Calbiochem?
储存条件	OK to freeze avoid repeated freeze/thaw cycles
运输	wet ice
储存温度	?70℃

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable