Endoglycosidase F1,Elizabethkingia meningosepticum, Recombinant,E. coli

半岛bd体育手机客户端 说明

一般描述

Recombinant,Elizabethkingia meningosepticumendoglycosidase F1 expressed inE. coli. Cleaves asparagine-linked or free oligomannose and hybrid, but not complex oligosaccharides. Core fucosylation reduces activity by 50 fold. Endo F1 will hydrolyze sulfate containing high mannose chains. It cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide generating a truncated sugar molecule with one N-acetylglucosamine residue remaining on the asparagine. Less sensitive to protein conformation than N-Glycosidase F (Cat. No. 362185) and therefore is more suitable for deglycosylation of native proteins.
Recombinant,Elizabethkingia meningosepticumendoglycosidase F1 expressed inE. coli. Cleaves asparagine-linked or free oligomannose and hybrid, but not complex oligosaccharides. Core fucosylation reduces activity by 50 fold. Endo F1 will hydrolyze sulfate containing high mannose chains. It cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide generating a truncated sugar molecule with one N-acetylglucosamine residue remaining on the asparagine. Less sensitive to protein conformation than N-Glycosidase F (Cat. No. 362185), and therefore is more suitable for deglycosylation of native proteins.
Note: 1 mU = 1 milliunit.

包装

700 miu in Glass bottle

警告

Toxicity: Standard Handling (A)

单位定义

One unit is defined as the amount of enzyme that will release N-linked oligosaccharides from 1.0 ?mol denatured ribonuclease B per min at 37°C, pH 5.5.

外形

In 20 mM Tris-HCl, pH 7.5.

其他说明

Tarentino, A.L., and Plummer, T.H. 1994.Methods Enzymol.230,44.
Tarentino, A.L., et al. 1992.J. Biol. Chem.267,3868.
Trimble, R.B., and Tarentino, A.L. 1991.J. Biol. Chem.266,1646.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

基本信息

酶学委员会(EC)编号

3.2.1.96 ( BRENDA | IUBMB )

半岛bd体育手机客户端 性质

质量水平	100
重组	expressed inE. coli
形式	liquid
specific activity	≥16 units/mg protein ≥17 units/mL
manufacturer/tradename	Calbiochem?
储存条件	do not freeze
异质活性	Proteases, none detected
运输	wet ice
储存温度	2-8℃

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable