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CDH13 human

品牌
Sigma-Aldrich
货号
5125
规格纯度
recombinant, expressed inE. coli, 0.5 mg protein/mL
价格
3673.63 *本价格含增值税费
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半岛bd体育手机客户端 名称:
CDH13 human
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

应用

Coating a plate well (6 well plate) with this recombinant CDH13 protein in a neuronal cell specific medium at 1-10 mg / well allows for 1) human neuronal cell and vascular endothelial cell / receptor interaction studies or 2) human neuronal and vascular endothelial cell/receptor interaction studies in vitro.

Use this procedure as a guideline to determine optimal coating conditions for the culture system of choice.
1. Thaw CDH13 and dilute to desired concentration using serum-free medium or PBS. The final solution should be sufficiently dilute so that the volume added covers the surface evenly (1-10 μg/well, 6 well plate).
2. Add appropriate amount of diluted material to culture surface.
3. Incubate at room temperature for approximately 1.5 hours.
4. Aspirate remaining material.
5. Rinse plates carefully with water and avoid scratching bottom surface of plates.
6. Plates are ready for use. They may also be stored at 2-8 ℃ damp or air dried if sterility is maintained.

序列

MASMTGGQQMGRGHHHHHHGNLYFQGGEFELSIVVSPILIPENQRQPFPRDVGKVVDSDRPERSKFRLTGKGVDQEPKGIFRINENTGSVSVTRTLDREVIAVYQLFVETTDVNGKTLEGPVPLEVIVIDQNDNRPIFREGPYIGHVMEGSPTGTTVMRMTAFDADDPATDNALLRYNIRQQTPDKPSPNMFYIDPEKGDIVTVVSPALLDRETLENPKYELIIEAQDMAGLDVGLTGTATATIMIDDKNDHSPKFTKKEFQATVEEGAVGVIVNLTVEDKDDPTTGAWRAAYTIINGNPGQSFEIHTNPQTNEGMLSVVKPLDYEISAFHTLLIKVENEDPLVPDVSYGPSSTATVHITVLDVNEGPVFYPDPMMVTRQEDLSVGSVLLTVNATDPDSLQHQTIRYSVYKDPAGWLNINPINGTVDTTAVLDRESPFVDNSVYTALFLAIDSGNPPATGTGTLLITLEDVNDNAPFIYPTVAEVCDDAKNLSVVILGASDKDLHPNTDPFKFEIHKQAVPDKVWKISKINNTHALVSLLQNLNKANYNLPIMVTDSGKPPMTNITDLRVQVCSCRNSKVDCNAAG

制备说明

The full-length extracellular domain of the humanCDH13gene (139-693 aa) was constructed with 31 N-terminal T7/HIS-tag and expressed inE. colias inclusion bodies. The final product was refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified.

基本信息

NACRES NA.75

半岛bd体育手机客户端 性质

质量水平 100
生物来源 human
重组 expressed inE. coli
描述 0.1 mg recombinant human CDH13 in 20 mM Tris-HCl buffer containing NaCl, KCl, EDTA, L-arginine, DTT and glycerol.
无菌性 Filtered sterilized solution
测定 ≥90% (SDS-PAGE)
形式 liquid
包装 pkg of 100 μg
浓度 0.5 mg protein/mL
technique(s) cell culture | mammalian: suitable
登记号 NP_001248
运输 dry ice
储存温度 ?20℃
Gene Information human ... CDH13(1012)

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK WGK 2
闪点(F) Not applicable
闪点(C) Not applicable

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