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CD86 human

品牌
Sigma-Aldrich
货号
5096
规格纯度
recombinant, expressed inE. coli, 0.5 mg protein/mL
价格
3733.99 *本价格含增值税费
促销
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数量
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半岛bd体育手机客户端 名称:
CD86 human
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

应用

Coating a plate well (6 well plate) with this recombinant CD86 protein in T cell specific medium at 1-10 μg/well allows for use as 1) a coating matrix protein for human T cell/ receptor interaction or as a highly purified recombinant antigen or 2) as a culture matrix protein for T cell differentiation regulation studiesin vitro.

Use this procedure as a guideline to determine optimal coating conditions for the culture system of choice.
1. Thaw CD86 and dilute to desired concentration using serum-free medium or PBS. The final solution should be sufficiently dilute so the volume added covers the surface evenly (1-10 μg/well, 6 well plate).
2. Add appropriate amount of diluted material to culture surface.
3. Incubate at room temperature for approximately 1.5 hours.
4. Aspirate remaining material.
5. Rinse plates carefully with water and avoid scratching bottom surface of plates.
6. Plates are ready for use. They may also be stored at 2-8 ℃ damp or air dried if sterility is maintained.

序列

MASMTGGQQMGRGHHHHHHGNLYFQGGEFELPLKIQAYFNETADLPCQFANSQNQSLSELVVFWQDQENLVLNEVYLGKEKFDSVHSKYMGRTSFDSDSWTLRLHNLQIKDKGLYQCIIHHKKPTGMIRIHQMNSELSVLANFSQPEIVPISNITENVYINLTCSSIHGYPEPKKMSVLLRTKNSTIEYDGIMQKSQDNVTELYDVSISLSVSFPDVTSNMTIFCILETDKTRLLSSPFSIELEDPQPPPDHIP

制备说明

The full-length extracellular domain of the human CD86 gene (24 - 247 aa, Isoform-2) was constructed with 31 N-terminal T7/HIS-tag and expressed inE. colias inclusion bodies. The final product was refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified as soluble protein.

基本信息

NACRES NA.75

半岛bd体育手机客户端 性质

生物来源 human
重组 expressed inE. coli
描述 0.1 mg recombinant human CD86 in 20 mM Tris-HCl buffer, containing NaCl, KCl, EDTA, L-arginine, DTT and glycerol.
无菌性 Filtered sterilized solution
测定 ≥90% (SDS-PAGE)
形式 liquid
包装 pkg of 100 μg
浓度 0.5 mg protein/mL
登记号 NP_008820
UniProt登记号 P42081
储存温度 ?20℃
Gene Information human ... CD86(942)

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK WGK 2
闪点(F) Not applicable
闪点(C) Not applicable

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