CD2 human

半岛bd体育手机客户端 说明

应用

Coating a plate well (6 well plate) with this recombinant CD2 protein in a T cell specific medium at 1-10 μg/well allows for human T cell differentiation studiesin vitro.

Use this procedure as a guideline to determine optimal coating conditions for the culture system of choice.
1. Thaw CD2 and dilute to desired concentration using serum-free medium or PBS. The final solution should be sufficiently dilute so that the volume added covers the surface evenly.
2. Add appropriate amount of diluted material to culture surface.
3. Incubate at room temperature for approximately 1.5 hours.
4. Aspirate remaining material.
5. Rinse plates carefully with water and avoid scratching bottom surface of plates.
6. Plates are ready for use. They may also be stored at 2-8 ℃ damp or air dried if sterility is maintained.

序列

MASMTGGQQMGRGHHHHHHGNLYFQGGEFELKEITNALETWGALGQDINLDIPSFQMSDDIDDIKWEKTSDKKKIAQFRKEKETFKEKDTYKLFKNGTLKIKHLKTDDQDIYKVSIYDTKGKNVLEKIFDLKIQERVSKPKISWTCINTTLTCEVMNGTDPELNLYQDGKHLKLSQRVITHKWTTSLSAKFKCTAGNKVSKESSVEPVSCPEKGLD

制备说明

The full-length extracellular domain of the humanCD2gene (25-209 aa) was constructed with 31 N-terminal T7/HIS-tag and expressed inE. colias inclusion bodies. The final product was refolded using a unique “temperature shift inclusion body refolding” technology and chromatographically purified.

基本信息

NACRES

NA.75

半岛bd体育手机客户端 性质

质量水平	100
生物来源	human
重组	expressed inE. coli
描述	0.1 mg recombinant human CD2 in 20 mM Tris-HCl buffer, pH 8.0, containing NaCl, KCl, EDTA, L -arginine, DTT, and glycerol
无菌性	Filtered sterilized solution
测定	≥90% (SDS-PAGE)
形式	liquid
包装	pkg of 100 μg
浓度	0.5 mg protein/mL
technique(s)	cell culture | mammalian: suitable
登记号	NP_001758
运输	dry ice
储存温度	?20℃
Gene Information	human ... CD2(914)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable