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位置: 首页> 品牌> Sigma-Aldrich> Anti-XRCC5 (Ku86) Antibody, clone N9C1

Anti-XRCC5 (Ku86) Antibody, clone N9C1

品牌
Sigma-Aldrich
货号
MABE1892
规格纯度
clone N9C1, from mouse
价格
1806.93 *本价格含增值税费
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数量
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半岛bd体育手机客户端 名称:
Anti-XRCC5 (Ku86) Antibody, clone N9C1
X-ray repair cross-complementing protein 5, 86 kDa subunit of Ku antigen, ATP-dependent DNA helicase 2 subunit 2, ATP-dependent DNA helicase II 80 kDa subunit, CTC box-binding factor 85 kDa subunit, CTC85, CTCBF, DNA repair protein XRCC5, Ku80, Ku86, Lup
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

X-ray repair cross-complementing protein 5 (UniProt: P13010; also known as 86 kDa subunit of Ku antigen, ATP-dependent DNA helicase 2 subunit 2, ATP-dependent DNA helicase II 80 kDa subunit, CTC box-binding factor 85 kDa subunit, CTC85, CTCBF,+D13 DNA repair protein XRCC5, Ku80, Ku86, Lupus Ku autoantigen protein p86, Nuclear factor IV, Thyroid-lupus autoantigen, TLAA, X-ray repair complementing defective repair in Chinese hamster cells 5) is encoded by the XRCC5 (also known as G22P2) gene (Gene ID: 7520) in human. XRCC5 is a single stranded DNA-dependent ATP-dependent helicase that exists as a heterodimer composed on XRCC5/XRCC6. This dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by about 100-fold. It plays a role in chromosome translocation. It binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It stabilizes broken DNA ends and bring them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ (Non-homologous end joining) ligation step. In association with NAA15, the XRCC5/6 dimer is shown to bind to the osteocalcin promoter and activates osteocalcin expression. The Ku domain of XRCC5 is localized in amino acids 251-260. It also contains an EEXXXDL motif (aa 720-728), which is required for the interaction with catalytic subunit PRKDC and its recruitment to sites of DNA damage. It undergoes phosphorylation on serine residues by PRKDC that enhances its helicase activity.

特异性

Clone N9C1 is a mouse monoclonal antibody that specifically detects DNA repair protein XRCC5.

免疫原

Full-length human recombinant DNA repair protein XRCC5 (Ku86).

应用

Western Blotting Analysis: A representative lot detected XRCC5 (Ku86) in Western Blotting applications (Knuth, M.W., et. al. (1990). J Biol Chem. 265(29):17911-20).

Anti-XRCC5 (Ku86), clone N9C1, Cat. No. MABE1892, is a highly specific mouse monoclonal antibody that targets DNA repair protein XRCC5 and has been tested for use in Western Blotting.
Research Category
Epigenetics & Nuclear Function

质量

Evaluated by Western Blotting in SW480 cell lysate.

Western Blotting Analysis: 1 ?g/mL of this antibody detected XRCC5 (Ku86) in SW480 cell lysate.

目标描述

~80 kDa observed; 82.71 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

外形

Protein G purified
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Format: Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss 32160702

半岛bd体育手机客户端 性质

生物来源 mouse
抗体形式 purified immunoglobulin
antibody product type primary antibodies
克隆 N9C1, monoclonal
species reactivity human
包装 antibody small pack of 25 μL
technique(s) western blot: suitable
同位素/亚型 IgG1λ
NCBI登记号 NP_066964
UniProt登记号 P13010

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK WGK 1
闪点(F) Not applicable
闪点(C) Not applicable

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