Anti-Vinculin Mouse mAb (V284 or VLN01)

半岛bd体育手机客户端 说明

一般描述

Protein G purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with mouse myeloma SP2/0 cells. Recognizes the ~130 (vinculin) and ~150 kDa (meta-vinculin) proteins.
Recognizes the ~130 (vinculin) and ~150 kDa (meta-vinculin) proteins in skin tissue.
This Anti-Vinculin Mouse mAb (V284 or VLN01) is validated for use in Immunoblotting, Immunoprecipitation, Paraffin Sections for the detection of Vinculin.

免疫原

semi-purified, human vinculin
Human

包装

100 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

应用

Immunoblotting (1 g/ml, chemiluminescence)
Immunoprecipitation (2 g/ml lysate)
Paraffin Sections (4-8 g/ml, heat pre-treatment required)

警告

Toxicity: Standard Handling (A)

外形

In 10 mM PBS, 0.2% BSA, pH 7.4.

分析说明

Negative Control
Normal mouse serum
Positive Control
Skin cells or tissue

其他说明

Palovuori, R., and Eskelinen, S. 2000Eur. J. Cell Biol.79,961.
Bakolitsa, C., et al. 1999Cell99,603.
Rudiger, M., et al. 1998Bioessays20,733
Tidball, J.G., et al. 1999J. Biol. Chem.274,33155
Goldmann, W.H., et al. 1996J. Muscle Res. Cell Motil.17,1.
Asijee, G.M., et al. 1990Eur. J. Biochemistry189,131
Otto, J.J. 1990Cell Motil. Cytoskeleton16,1.
This antibody is useful for studies of the role of vinculin in platelet activation. Vinculin (Ab-1) exhibits membrane and cytoplasmic staining when used in immunohistochemistry. For staining of formalin-fixed paraffin sections, boil in 10 mM citrate buffer, pH 6.0, for 10-20 min, followed by cooling at room temperature for 20 min. Antibody should be titrated for optimal results in individual systems.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified antibody
antibody product type	primary antibodies
克隆	V284, monoclonal VLN01, monoclonal
形式	liquid
包含	≤0.1% sodium azide as preservative
species reactivity	human
manufacturer/tradename	Calbiochem?
储存条件	do not freeze
同位素/亚型	IgG1
运输	wet ice
储存温度	2-8℃

安全信息

储存分类代码	11 - Combustible Solids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable