半岛bd体育手机客户端 说明
一般描述
ATP-dependent DNA helicase Q1 (UniProt: P46063; also known as EC:3.6.4.12, DNA helicase, RecQ-like type 1, RecQ1, DNA-dependent ATPase Q1, RecQ protein-like 1) is encoded by the RECQL (also known as RECQ1, RECQL1) gene (Gene ID: 5965) in human. RecQ helicases are a ubiquitous family of DNA unwinding enzymes involved in the maintenance of chromosome stability. They participate in various types of DNA repair, including mismatch repair, nucleotide excision repair and direct repair. Five members of the RecQ family have been reported in human cells: BLM, RECQ1, RECQ4, RECQ5, and WRN. RecQ1 displays high expression in heart, lung, skeletal muscle and kidney. It is also highly expressed in tumor cells and although brain exhibits low level of EecQ1, its expression is dramatically increased in human glioblastoma. Depletion of RecQ1 affects proliferation of glioblastoma cells and causes an increase in the level of DNA damage. RecQ1 depleted tumor cells are shown to be more sensitive to anti-tumor agents, such as hydroxyurea and temozolomide.
特异性
Clone 5A12.1 specifically detects human ATP-dependent DNA helicase Q1 (RecQ1). It targets an epitope within 87 amino acids from the internal region.
免疫原
GST/His-tagged recombinant fragment corresponding to 87 amino acids from the internal region of human ATP-dependent DNA helicase Q1.
应用
Anti-RecQ1, clone 5A12.1, Cat. No. MABE1055, is a highly specific mouse monoclonal antibody that targets RecQ1 and has been tested for use in Western Blotting and Immunohistochemistry (Paraffin).
Immunohistochemistry (Paraffin) Analysis: A 1:50-250 dilution from a representative lot detected RecQ1 in human kidney and human tonsil tissue sections.
质量
Evaluated by Western Blotting in Raji cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected RecQ1 in Raji cell lysate.
目标描述
~73 kDa observed; 73.46 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
外形
Protein G purified
Format: Purified
其他说明
Concentration: Please refer to lot specific datasheet.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160702 |
半岛bd体育手机客户端 性质
| 生物来源 | mouse |
| 抗体形式 | purified immunoglobulin |
| antibody product type | primary antibodies |
| 克隆 | 5A12.1, monoclonal |
| species reactivity | human |
| 包装 | antibody small pack of 25 μL |
| technique(s) | immunohistochemistry: suitable (paraffin) western blot: suitable |
| 同位素/亚型 | IgG2aκ |
| NCBI登记号 | NP_002898 |
| UniProt登记号 | P46063 |
Sigma-Aldrich
m.cnreagent.com
