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位置: 首页> 品牌> Sigma-Aldrich> Anti-PGM5 (Aciculin) Antibody, clone 14F8

Anti-PGM5 (Aciculin) Antibody, clone 14F8

品牌
Sigma-Aldrich
货号
MABT1503
规格纯度
clone 14F8, from mouse
价格
1415.25 *本价格含增值税费
促销
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数量
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半岛bd体育手机客户端 名称:
Anti-PGM5 (Aciculin) Antibody, clone 14F8
Aciculin, Phosphoglucomutase-like Protein 5, PGM-RP
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Phosphoglucomutase-like protein 5 (UniProt: Q15124; also known as Aciculin, Phosphoglucomutase-related protein, PGM-RP) is encoded by the PGM5 (also known as PGMRP) gene (Gene ID: 5239) in human. Aciculin is a dystrophin and utrophin-binding protein that is highly homologous to the glycolytic enzyme phosphoglucomutase type 1. However, it lacks phosphoglucomutase activity. Aciculin is a component of adherens-type cell-cell and cell-matrix junctions and is concentrated in focal contacts at the ends of actin bundles and associates with actin filaments. Aciculin is also reported to be an interaction partner of Filamin C and Xin proteins. Higher levels of Aciculin are reported in smooth and cardiac muscle and lower levels are present in skeletal muscle, liver, kidney and brain tissue. In striated muscle, it mainly localizes to the intercalated discs of the heart and to myotendinous junctions. Low levels of Aciculin are expressed in 10-12-week-old fetus and these levels increase progressively into infancy reaching maximum levels at maturity. Two isoforms of Aciculin have been reported that are produced by alternative splicing. Aciculin knockdown in myotubes is shown to cause failure in myofibril assembly, alignment and membrane attachment, and a massive reduction in myofibril number. (Ref.: Molt, S., et al., (2014). J. Cell Sci.127(16); 3578-3592).

特异性

Clone 14F8 detects Phosphoglucomutase-like protein 5 in mouse and rat cardiac tissue and is predicted to react with human.

免疫原

Cytoskeletal fraction of the human myometrium tissue extract.

应用

Research Category
Cell Structure
Western Blotting Analysis: 1 ?g/mL from a representative lot detected PGM5 (Aciculin) in rat heart tissue lysate.

Immunoprecipitation Analysis: A representative lot immunoprecipitated PGM5 (Aciculin) in Immunoprecipitation applications (Belkin, A.M., et. al. (1995). J Biol Chem. 270(11):6328-37; Belkin, A.M., et. al. (1995). Exp Cell Res. 221(1):132-40).

Immunofluorescence Analysis: A representative lot detected PGM5 (Aciculin) in Immunofluorescence applications (Belkin, A.M., et. al. (1994). J Cell Sci. 107 ( Pt 7):1993-2003).

Immunohistochemistry Analysis: A representative lot detected PGM5 (Aciculin) in Immunohistochemistry applications (Belkin, A.M., et. al. (1995). Exp Cell Res. 221(1):132-40).

Western Blotting Analysis: A representative lot detected PGM5 (Aciculin) in Western Blotting applications (Belkin, A.M., et. al. (1995). J Biol Chem. 270(11):6328-37; Belkin, A.M., et. al. (1994). J Cell Sci. 107 ( Pt 7):1993-2003).

ELISA Analysis: A representative lot detected PGM5 (Aciculin) in ELISA applications (Molt, S., et. al. (2014). J Cell Sci. 127(Pt 16):3578-92).

Anti-PGM5 (Aciculin), clone 14F8, Cat. No. MABT1503, is a mouse monoclonal antibody that detects Phosphoglucomutase-like protein 5 and has been tested for use in ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, and Western Blotting.

质量

Evaluated by Western Blotting in mouse heart tissue lysate.

Western Blotting Analysis: 1 ?g/mL of this antibody detected PGM5 (Aciculin) in mouse heart tissue lysate.

目标描述

~57 kDa observed; 62.22 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

外形

Protein G purified
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Format: Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss 32160702
NACRES NA.41

半岛bd体育手机客户端 性质

生物来源 mouse
抗体形式 purified antibody
antibody product type primary antibodies
克隆 14F8, monoclonal
species reactivity human, rat, rabbit, bovine, mouse
species reactivity (predicted by homology) porcine, frog, avian
包装 antibody small pack of 25 μg
technique(s) ELISA: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
同位素/亚型 IgG1κ
NCBI登记号 NP_068800
UniProt登记号 Q15124
Gene Information human ... PGM5(5239)

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK WGK 1
闪点(F) Not applicable
闪点(C) Not applicable

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