Anti-p21^WAF1(Ab-1) Mouse mAb (EA10)

半岛bd体育手机客户端 说明

一般描述

This Anti-p21^WAF1(Ab-1) Mouse mAb (EA10) is validated for use in FC, Immunoblotting, IF, IP, Paraffin Sections for the detection of p21^WAF1(Ab-1).
Recognizes the ~21 kDa p21^WAF1protein in skin and colon tissue and in cells expressing wild-type p53 (e.g. Hs27 or U205 cells treated with DNA damaging agents).

包装

20, 100 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

应用

Flow Cytometry (2 g/ml or use Cat. No. OP64F; see application references)
Frozen Sections (5 g/ml or use Cat. No. OP64F)
Immunoblotting (1-3 g/ml)
Immunofluorescence (1-5 g/ml or use Cat. No. OP64F)
Immunoprecipitation (2 g/sample)
Paraffin Sections (5 g/ml or use OP64F; heat pre-treatment required; see comments and application references)

警告

Toxicity: Standard Handling (A)

分析说明

Positive Control
Any cell line expressing wild-type p53 (e.g. Hs27 or U2OS treated with DNA-damaging agents) or skin or colon tissue

其他说明

Agarwal, M.L., et al. 1995.Proc. Natl. Acad. Sci. USA92,8493.
Chen, Y.Q., et al. 1995.Int. J. Oncology7,889.
Deng, C., et al. 1995.Cell82,675.
El-Deiry, W.S., et al. 1995.Cancer Res.55,2910.
Waldman, T., et al. 1995.Cancer Res.55,5187.
Elbendary, A., et al.1994.Cell Growth Diff.5,1301.
El-Deiry, W.S., et al. 1994Cancer Res.54,1169.
Li, R., et al. 1994.Nature371,534.
Michieli, P., et al. 1994.Cancer Res.54,3391.
Noda, A., et al.1994.Exp. Cell Res.211,90.
El-Deiry, W.S., et al.1993.Cell75,817.
Gu, Y., et al. 1993.Nature366,707.
Harper, J.W., et al.1993.Cell75,805.
Xiong, Y., et al.1993.Genes Devel.7,1572.
Xiong, Y., et al.1993.Nature366,701.
Xiong, Y., et al.1992.Cell71,505.
Maximal p21WAF1 expression requires wild type p53 activity. Treatment of U2OS or MCF7 cells with DNA damaging agents (such as doxorubicin at 0.2 ?g/ml) induces wild type p53 expression which in turn activates WAF1 expression. Serum stimulation of quiescent cells will give low level WAF1 expression independent of p53 expression. Untreated cells will express little p21WAF1 and can be used as a negative control. Cat. No. OP64F was tested in HALT cells induced by incubation at 31°C; FITC-goat anti-mouse IgG (Cat. No. DC13L) was used as a negative control. This antibody will immunoprecipitate p21WAF1 but not associated proteins. For immunoblotting applications, use a 0.22 ?m filter and visualize by chemiluminescence. For staining paraffin sections, heating the tissue in 10 mM citrate buffer is required (see application references). In either paraffin or frozen sections of normal human colon, the non-dividing cells of colonic epithelium will stain positive for p21WAF1 while the proliferating compartment of crypts will not stain. Antibody should be titrated for optimal results in individual systems.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified antibody
antibody product type	primary antibodies
克隆	EA10, monoclonal
形式	liquid
包含	≤0.1% sodium azide as preservative
species reactivity	human
should not react with	rat, mouse
manufacturer/tradename	Calbiochem?
储存条件	do not freeze
同位素/亚型	IgG1
运输	wet ice
储存温度	2-8℃

安全信息

储存分类代码	11 - Combustible Solids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable