Anti-p53 (Ab-6) (Pantropic) Mouse mAb (DO-1)

半岛bd体育手机客户端 说明

一般描述

Recognizes the ~53 kDa wild-type and mutant p53 protein in A431 cells and breast carcinoma tissue.
This Anti-p53 (Ab-6) (Pantropic) Mouse mAb (DO-1) is validated for use in Frozen sections, Immunoblotting, ICC, IP, Paraffin sections for the detection of p53 (Ab-6) (Pantropic).
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with X63Ag8.653 mouse myeloma cells (see application references). Recognizes the ~53 kDa wild-type and mutant forms of p53.

免疫原

wild type, recombinant, human p53
Epitope: Within amino acids 21-25 of human p53
Human

包装

20, 100 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

应用

Frozen sections (1 g/ml; see application references)
Immunoblotting (0.1-1 g/ml; see application references)
Immunocytochemistry (1-2.5 g/ml; see application references)
Immunoprecipitation (1 g/ml or use Cat. No. OP43A; see application references)
Paraffin sections (1 g/ml, pepsin, heat or pressure cooker pre-treatment required; see application references)

警告

Toxicity: Standard Handling (A)

外形

In 0.05 M sodium phosphate buffer, 0.2% gelatin.

分析说明

Negative Control
SK-OV-3 cells or normal skin tissue
Positive Control
A431 cells or breast carcinoma tissue

其他说明

El-Deiry, W.S., et al. 1994.Cancer Res.54,1169.
Greenblatt, M.S., et al. 1994.Cancer Res.54,4855.
Legros, Y., et al. 1994.Oncogene9,2071.
Barak, Y., et al. 1993.EMBO J.12,461.
Kastan, M.B., et al. 1992.Cell71,587.
Kuerbitz, S.J. 1992.Proc. Natl. Acad. Sci. USA89,7491.
Lane, D.P. 1992.Nature358,15.
Vojtesek, B., et al. 1992.J. Immunol. Meth.151,237.
Kastan, M.B., et al. 1991Cancer Res.51,6304.
Recognizes both mutant and wild-type p53 under denaturing and non-denaturing conditions. This antibody reacts weakly with rodent p53; we do not recommend it for rodent samples. For gel shift assay, use Cat. No. OP43L resuspended in 100 μl of buffer. Wild-type p53 has a short half life and is present in low amounts in cells. For immunoprecipitation increasing the amount of sample and labeling with³⁵S-Met for less than or equal to 1 h will aid in visualizing wild-type p53. Antibody should be titrated for optimal results in individual systems.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified antibody
antibody product type	primary antibodies
克隆	DO-1, monoclonal
形式	liquid
包含	≤0.1% sodium azide as preservative
species reactivity	feline, human
should not react with	mouse, rat
manufacturer/tradename	Calbiochem?
储存条件	do not freeze
同位素/亚型	IgG2a
运输	wet ice
储存温度	2-8℃

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	nwg
闪点(F)	Not applicable
闪点(C)	Not applicable