Anti-p53 (Ab-3) (Mutant) Mouse mAb (PAb240)

半岛bd体育手机客户端 说明

一般描述

This Anti-p53 (Ab-3) (Mutant) Mouse mAb (PAb240) is validated for use in FC, Frozen Sections, Gel Shift, Immunoblotting, IF, IP, Paraffin Sections for the detection of p53 (Ab-3) (Mutant).
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2 mouse myeloma cells (see application references). Recognizes the ~53 kDa mutant p53 protein under non-denaturing conditions. Recognizes both the mutant and the wild-type p53 protein under denaturing conditions.
Recognizes the ~53 kDa mutant p53 protein under non-denaturing conditions by immunoprecipitation, immunofluorescence, and flow cytometry. Recognizes both mutant and wild-type p53 by immunoblotting and paraffin sections under denaturing conditions.

免疫原

Human
Epitope: within amino acids 213-217
a recombinant protein consisting of amino acids 14-389 of p53 fused to β-galactosidase

包装

100, 200 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

应用

Flow Cytometry (1-20 ?g/ml)

Frozen Sections (10 ?g/ml)

Gel Shift (see comments)

Immunoblotting (5 ?g/ml)

Immunofluorescence (1-20 ?g/ml, see application references)

Immunoprecipitation (1 ?g per sample)

Paraffin Sections (see application references)

警告

Toxicity: Standard Handling (A)

外形

In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.

分析说明

Negative Control
SK-OV-3 cells
Positive Control
A431, Hs27 (wild-type p53), or SK-BR-3 cells or breast carcinoma tissue

其他说明

El-Deiry, W.S., et al. 1994.Cancer Res.54,1169.
Greenblatt, M.S., et al. 1994.Cancer Res.54,4855.
Barak, Y., et al. 1993.EMBO J.12,461.
Kastan, M.B., et al. 1992.Cell71,587.
Kuerbitz, S.J. 1992.Proc. Natl. Acad. Sci. USA89,7491.
Lane, D.P. 1992.Nature358,15.
Kastan, M.B., et al. 1991.Cancer Res.51,6304.
Under non-denaturing conditions (immunoprecipitation, immunofluorescence and frozen sections), Anti-p53 (Ab-3) does not recognize normal (wild-type) p53 protein; it recognizes an epitope exposed by activating mutations or denaturation. In denaturing protocols (immunoblotting and paraffin sections), Anti-p53 (Ab-3) will recognize both mutant and wild-type p53. Will not recognize to some p53 molecules with mutations in the RHSVV epitope, but will react to TFIIIA, which has the RHSVV epitope. For gel shift assay, use Cat. No. OP29L and resuspend in 100 ?l buffer. Antibody should be titrated for optimal results in individual systems.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified antibody
antibody product type	primary antibodies
克隆	PAb240, monoclonal
形式	liquid
包含	≤0.1% sodium azide as preservative
species reactivity	hamster, chicken, mouse, bovine, human, rat
should not react with	Xenopus
manufacturer/tradename	Calbiochem?
储存条件	do not freeze
同位素/亚型	IgG1
运输	wet ice
储存温度	2-8℃

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	nwg
闪点(F)	Not applicable
闪点(C)	Not applicable