Anti-p53 (Ab-2) (Pantropic) Mouse mAb (PAb1801)

半岛bd体育手机客户端 说明

一般描述

Recognizes the ~53 kDa wild-type and mutant p53 protein in A-431 cells and breast carcinoma tissue.
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2/0 Ag14 mouse myeloma cells (see application references). Recognizes the ~53 kDa mutant and wild-type forms of p53.
This Anti-p53 (Ab-2) (Pantropic) Mouse mAb (PAb1801) is validated for use in Frozen Sections, Gel Shift, Immunoblotting, IP, Paraffin Sections for the detection of p53 (Ab-2) (Pantropic).

免疫原

a human p53 fusion protein
Epitope: within amino acids 46-55 of human p53
Human

包装

100, 200 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

应用

Frozen Sections (5 ?g/ml)

Gel Shift (see comments)

Immunoblotting (2.5 ?g/ml)

Immunoprecipitation (1 ?g/sample)

Paraffin Sections (5 ?g/ml, pepsin, trypsin, or heat pre-treatment required)

警告

Toxicity: Standard Handling (A)

外形

In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.

分析说明

Positive Control
A431 cells or breast carcinoma tissue
Negative Control
SK-OV-3 cells or normal skin

其他说明

Wild-type p53 has a short half-life and is present in low amounts in cells. For immunoprecipitation, increasing the amount of sample to be immunoprecipitated and applied to the gel may help visualize wild-type p53; short incubation times with³⁵S-Met (≤ 1 h) will help reduce background. For immunoblots of wild-type p53, maximize sensitivity by preconcentrating samples by immunoprecipitation with Cat. No. OP09, then immunoblot using Cat. No. PC35 and chemiluminescent detection. For a gel shift assay, use Cat. No. OP09L and resuspend in 100 ?l buffer. Antibody should be titrated for optimal results in individual systems.
El-Deiry, W.S., et al. 1994.Cancer Res.54,1169.
Greenblatt, M.S., et al. 1994.Cancer Res.54,4855.
Barak, Y., et al. 1993.EMBO J.12,461.
Kastan, M.B., et al. 1992.Cell71,587.
Kuerbitz, S.J. 1992.Proc. Natl. Acad. Sci. USA89,7491.
Lane, D.P. 1992.Nature358,15.
Kastan, M.B., et al. 1991.Cancer Res.51,6304.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified antibody
antibody product type	primary antibodies
克隆	PAb1801, monoclonal
形式	liquid
包含	≤0.1% sodium azide as preservative
species reactivity	human
should not react with	mouse, rat
manufacturer/tradename	Calbiochem?
储存条件	do not freeze
同位素/亚型	IgG1
运输	wet ice
储存温度	2-8℃

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	nwg
闪点(F)	Not applicable
闪点(C)	Not applicable