Anti-p53 (Ab-1) (Pantropic) Mouse mAb (PAb421)

半岛bd体育手机客户端 说明

一般描述

Recognizes the ~53 kDa mammalian wild-type and mutant p53 protein. Does not recognize phosphorylated or acetylated p53.
This Anti-p53 (Ab-1) (Pantropic) Mouse mAb (PAb421) is validated for use in Frozen Sections, Gel Shift, Immunoblotting, IF, FC, IP for the detection of p53 (Ab-1) (Pantropic).
Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS1 mouse myeloma cells (see application references). Recognizes the ~53 kDa mutant and wild-type p53 proteins.

免疫原

partially purified mouse p53
Mouse
Epitope: within amino acids 376-378 of human p53

包装

20, 100 μg in Glass bottle
Please refer to vial label for lot-specific concentration.

应用

Frozen Sections (10 ?g/ml, see application references)

Gel Shift (see comments)

Immunoblotting (10 ?g/ml, see application references)

Immunofluorescence (1-20 ?g/ml, see application references)

Flow Cytometry (1-20 ?g/ml)

Immunoprecipitation (1 μg per sample, see application references)

警告

Toxicity: Standard Handling (A)

外形

In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.

分析说明

Negative Control
SK-OV-3 cells or normal skin
Positive Control
Breast carcinoma, A431 cells, or p53 standard (Cat. No. 506147)

其他说明

Originally called clone L21. Does not recognize phosphorylated p53. For a gel shift assay, use Cat. No. OP03L and resuspend in 100 ?l buffer. Wild-type p53 has a short half-life and is present in low amounts in cells. For immunoprecipitation, increasing the amount of sample to be immunoprecipitated and applied to the gel may help visualize wild-type p53, and short incubation times with³⁵S-Met (≤1 h) will help reduce background. For immunoblotting of wild-type p53, maximize sensitivity by concentrating samples by immunoprecipitation with Cat. No. OP03, then immunoblot using PC35 and chemiluminescent detection. This antibody recognizes the region encompassing Ser³⁷⁶to Ser³⁷⁸(see application references) and does not recognize phosphorylated p53 (see application references). Antibody should be titrated for optimal results in individual systems.
El-Deiry, W.S., et al. 1994.Cancer Res.54,1169.
Greenblatt, M.S., et al. 1994.Cancer Res.54,4855.
Legros, Y., et al. 1994.Oncogene9,2071.
Barak, Y., et al. 1993.EMBO J.12,461.
Kuerbitz, S.J. 1992.Proc. Natl. Acad. Sci. USA89,7491.
Kastan, M.B., et al. 1992.Cell71,587.
Lane, D.P. 1992.Nature358,15.
Kastan, M.B., et al. 1991.Cancer Res.516304.
Crawford, L. and Harlow, E. 1982.J. Virol.41,709.
Harlow, E., et al. 1981.J. Virol.39861.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified antibody
antibody product type	primary antibodies
克隆	PAb421, monoclonal
形式	liquid
包含	≤0.1% sodium azide as preservative
species reactivity	mouse, rabbit, monkey, rat, human
manufacturer/tradename	Calbiochem?
储存条件	do not freeze
同位素/亚型	IgG2a
运输	wet ice
储存温度	2-8℃

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	nwg
闪点(F)	Not applicable
闪点(C)	Not applicable