Anti-MSH2 (Ab-1) Mouse mAb (GB12)

半岛bd体育手机客户端 说明

一般描述

Recognizes the ~100 kDa MSH2 protein in HCT116 and SW480 cells and colon tissue.
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP20 mouse myeloma cells. Recognizes the ~100 kDa MSH2 protein.
This Anti-MSH2 (Ab-1) Mouse mAb (GB12) is validated for use in Frozen Sections, Immunoblotting, Immunoprecipitation, Paraffin Sections for the detection of MSH2 (Ab-1).

免疫原

Human
an N-terminal fragment of human MSH2

包装

100 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

应用

Frozen Sections (5 ?g/ml)

Immunoblotting (1 ?g/ml)

Immunoprecipitation (1 ?g/reaction)

Paraffin Sections (3 ?g/ml, heat pre-treatment required)

警告

Toxicity: Standard Handling (A)

外形

In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.

分析说明

Positive Control
HCT116 or SW480 cells or colon tissue
Negative Control
LoVo cells

其他说明

Bronner, C. E., et al. 1994.Nature368,258.
Papadopoulos, N., et al. 1994.Science263,1625.
Peltom?ki, P. T. 1994.Annals of Medicine26,215.
Fishel, R., et al. 1993.Cell75,1027-1038, 1993.
Leach, F. S., et al. 1993.Cell75,1215.
Lindbolm, A., et al. 1993.Nature Genetics5,279.
For paraffin sections, pre-treat with heat for 20 min. Antibody should be titrated for optimal results in individual systems.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified antibody
antibody product type	primary antibodies
克隆	GB12, monoclonal
形式	liquid
包含	≤0.1% sodium azide as preservative
species reactivity	human
should not react with	mouse
manufacturer/tradename	Calbiochem?
储存条件	do not freeze
同位素/亚型	IgG1
运输	wet ice
储存温度	2-8℃

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	nwg
闪点(F)	Not applicable
闪点(C)	Not applicable