Anti-LDL Receptor Mouse mAb (15C8)

半岛bd体育手机客户端 说明

一般描述

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with Sp2/0-Ag14 myeloma cells (see application references). Recognizes the ~160 kDa LDL Receptor protein.
Recognizes the ~160 kDa LDL receptor protein in HT1080 and HepG2 cells.
This Anti-LDL Receptor Mouse mAb (15C8) is validated for use in ELISA, Flow Cytometry, Frozen Sections, Immunoblotting, Immunocytochemistry, Immunoprecipitation for the detection of LDL Receptor.

免疫原

Bovine
a trypic digest of bovine low density lipoprotein receptor
Epitope: first cysteine-rich ligand binding domain

包装

100 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

应用

ELISA (0.5-1 g/ml)
Flow Cytometry (see application references)
Frozen Sections (1-5 g/ml)
Immunoblotting (1-5 g/ml, see application references)
Immunocytochemistry (1-5 g/ml, IF)
Immunoprecipitation (1-2 g/sample)

警告

Toxicity: Standard Handling (A)

外形

In 0.05 M sodium phosphate buffer, 0.2% gelatin.

分析说明

Positive Control
Liver sections, HT1080 cells, HepG2 cells

其他说明

Will displace 50-70% of LDL binding to the receptor. For immunoblotting, include 2 mM Ca⁺²with the antibody and avoid EDTA. This antibody has been reported to bind best to the unreduced LDL-R (see specificity application reference). Antibody should be titrated for optimal results in individual systems.
van Driel, I.R., et al. 1987.J. Biol. Chem.262,16127.
van Driel, I.R., et al. 1987.J. Biol. Chem.262,17443.
Russell, D.W., et al. 1984.Cell37,577.
Achneider, W.J., et al. 1983.J. Cell Biol.97,1635.
Cummings, R.D., et al. 1983.J. Biol. Chem.258,15261.
Schneider, W.J., et al. 1982J. Biol. Chem.257,2664.
Goldstein, J.L., et al. 1979.Nature279,679.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified antibody
antibody product type	primary antibodies
克隆	15C8, monoclonal
形式	liquid
包含	≤0.1% sodium azide as preservative
species reactivity	bovine, human
should not react with	rabbit
manufacturer/tradename	Calbiochem?
储存条件	do not freeze
同位素/亚型	IgG2a
运输	wet ice
储存温度	2-8℃

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	nwg
闪点(F)	Not applicable
闪点(C)	Not applicable