Anti-Histidine-Tagged Protein Mouse mAb (13/45/31/2)

半岛bd体育手机客户端 说明

一般描述

Anti-Histidine-Tagged Protein, mouse monoclonal, clone 13/45/31/2, specifically recognizes an epitage of 6 consecutive His residues. It is validated for use in ELISA, WB, IF & IP.
Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with Sp2/0 mouse myeloma cells. Recognizes a His?Tag sequence in native and recombinant proteins. Specifically recognizes an epitope of six consecutive His residues of both natural and recombinant sources.
Specifically recognizes an epitage of six consecutive His residues of both natural and recombinant sources.

免疫原

Epitope: HHHHHH
a recombinant protein containing a His?Tag sequence

包装

100 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

应用

ELISA (0.1-0.4 g/ml)
Immunoblotting (1-4 g/ml)
Immunofluorescence (1-4 g/ml)
Immunoprecipitation (4 g/ml)

警告

Toxicity: Standard Handling (A)

外形

In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.

分析说明

Positive Control
Proteins containing a His?Tag sequence

其他说明

Zentgraf, H., et al. 1995.Nucl. Acids. Res.23, 3347.
Gu, J., et al. 1994.BioTechniques17, 257.
Sporeno, E., et al. 1994.J. Biol. Chem., 10991.
Sisk, W.P., et al. 1994.J. Virol.68, 766.
Lu, T., et al. 1993.Anal. Biochem.213, 318.
Garner, J., et al. 1992.Cell69, 833.
Hochuli, E., et al. 1987.J. Chromatogr.411, 177.
Antibody should be titrated for optimal results in individual systems.

法律信息

Manufactured by Dianova, GmbH, Germany.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified antibody
antibody product type	primary antibodies
克隆	13/45/31/2, monoclonal
形式	liquid
包含	≤0.1% sodium azide as preservative
species reactivity (predicted by homology)	all
manufacturer/tradename	Calbiochem?
储存条件	do not freeze
同位素/亚型	IgG1
运输	wet ice
储存温度	2-8℃

安全信息

储存分类代码	11 - Combustible Solids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable