半岛bd体育手机客户端 说明
一般描述
Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells. Recognizes the ~170 kDa EGF receptor protein.
Anti-EGFR (Ab-5), mouse monoclonal, clone EGFR.1, recognizes the ~170 kDa EGF receptor protein in A431 cells. It is validated for use in FC, IF, and IP.
Recognizes the ~170 kDa EGF receptor protein in A431 cells. For neutralization studies Cat. No. GR15L is recommended.
免疫原
partially purified, human EGFR from A431 cells
Human
包装
100 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.
应用
Flow Cytometry (1-5 g/ml)
Immunofluorescence (1-5 g/ml)
Immunoprecipitation (1-2 g/sample)
Neutralization Studies (1-2 g/ml; use Cat. No. GR15L; see application references)
警告
Toxicity: Standard Handling (A)
外形
In 50 mM sodium phosphate, 0.2% gelatin, pH 7.5.
分析说明
Negative Control
HL-60 cells
Positive Control
A-431 cells
其他说明
EGF-Receptor (Ab-5) binds to A431, HeLa-5 cells and other human cells but not to rodent cells. It inhibits EGF binding to its receptor and is an antagonist ofin vivoEGF-stimulated tyrosine kinase activity. Does not cross-react to c-neu/erbB-2. Antibody should be titrated for optimal results in individual systems.
Hunter T., 1984.Nature311,414.
Reynolds, F.H., Jr., et al. 1981.Nature292,259.
Ushiro, H. and Cohen, S. 1980.J. Biol. Chem.255,8363.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
半岛bd体育手机客户端 性质
| 质量水平 | 100 |
| 生物来源 | mouse |
| 抗体形式 | purified antibody |
| antibody product type | primary antibodies |
| 克隆 | EGFR.1, monoclonal |
| 形式 | liquid |
| 包含 | ≤0.1% sodium azide as preservative |
| species reactivity | human |
| should not react with | rat, mouse |
| manufacturer/tradename | Calbiochem? |
| 储存条件 | do not freeze |
| 同位素/亚型 | IgG2b |
| 运输 | wet ice |
| 储存温度 | 2-8℃ |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | nwg |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
