Anti-CD64 Mouse mAb (10.1)

半岛bd体育手机客户端 说明

一般描述

Recognizes the ~72 kDa CD64 protein (FcR1 receptor).
Anti-CD64, mouse monoclonal, clone 10.1, recognizes the ~72 kDa CD64 protein (FcR1). It is validated for FC, WB, IP, IHC on frozen sections, and for neutralization studies.
Purified mouse monoclonal antibody. Recognizes the ~72 kDa CD64 (FcR1) protein.

免疫原

human rheumatoid synovial fluid cells/monocytes
Human

包装

100 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

应用

Flow Cytometry (10 ?g/ml)

Frozen Sections (see comments)

Immunoblotting (see comments)

Immunoprecipitation (see comments)

Neutralization Studies (see comments)

警告

Toxicity: Standard Handling (A)

外形

In 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.

重悬

Following initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.

分析说明

Negative Control
HPB-MLT
Positive Control
4937 cells

其他说明

The antibody is reported to block immunoglobulin binding to FcR1. FACS was performed on 5x10⁵cultured U-937 human tumor cells and analyzed by flow cytometry. Optimal binding concentration tested was 10 ?g/ml when used with secondary reagent goat anti-mouse IgG, FITC conjugated. This antibody has also been reported to work for frozen sections, immunoblotting, and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
Dougherty, G.J., et al. 1987.Eur. J. Immunol.17,1453.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified antibody
antibody product type	primary antibodies
克隆	10.1, monoclonal
测定	≥95% (SDS-PAGE)
形式	liquid
包含	0.5 mg/mL gentamycin sulfate as preservative
species reactivity	human
manufacturer/tradename	Calbiochem?
储存条件	OK to freeze
同位素/亚型	IgG1
运输	wet ice
储存温度	2-8℃

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable