Anti-Cathepsin D (Ab-1) Mouse mAb (BC011)

半岛bd体育手机客户端 说明

一般描述

Recognizes the ~52 kDa latent form, the ~48 kDa mature form, and the ~34 kDa subunit of cathepsin D in breast carcinoma tissue extracts.
Purified mouse monoclonal antibody generated by immunizing CB6F1 mice with the specified immunogen and fusing splenocytes with SP2/0 cells. Recognizes the ~52 kDa pro-cathespin D, the ~48 kDa mature cathepsin D, and the ~34 kDa subunit of cathespin D.
Anti-Cathepsin D (Ab-1), mouse monoclonal, clone BC011, recognizes latent and mature form and the ~34 kDa subunit in breast carcinoma tissue extracts. It is validated for WB and for paraffin sections.

免疫原

mature, human cathepsin D

包装

100 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

应用

Immunoblotting (1 ?g/ml)

Paraffin Sections (5 ?g/ml, trypsin pre-treatment required)

警告

Toxicity: Standard Handling (A)

其他说明

Rochefort, H. 1992.Eur. J. Cancer.28A,1780.
Rochefort, H. 1990. inSeminars in Cancer Biology, ed. M.M. Gottesman.Vol. 1(2),153.
Westly, B. and Rochefort, H. 1980.Cell20,352.
Barrett, A.J. 1977. inProteinases in Mammalian Cells and Tissues, ed. Barret, A.J. (North Holland, New York), pp. 209.
Does not detect the ~14 kDa subunit of cathespin D, nor will it detect cathespins B or L. Cathepsins are secreted and accumulate in conditioned tissue culture media and can be detected by immunoblotting from this source. Some cell extracts do not contain sufficient cathepsin for detection by immunoblotting. Antibody should be titrated for optimal results in individual systems.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified antibody
antibody product type	primary antibodies
克隆	BC011, monoclonal
形式	liquid
包含	≤0.1% sodium azide as preservative
species reactivity	human
manufacturer/tradename	Calbiochem?
储存条件	do not freeze
同位素/亚型	IgG2a
运输	wet ice
储存温度	2-8℃

安全信息

储存分类代码	11 - Combustible Solids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable