Anti-BRCA1 (Ab-2) Mouse mAb (MS13)

半岛bd体育手机客户端 说明

一般描述

Purified mouse monoclonal antibody generated by immunizing mice with the specified iummunogen and fusing splenocytes with NS1 mouse myeloma cells. Recognizes the ~220 kDa BRCA1 protein.
Anti-BRCA1 (Ab-2), mouse monoclonal, clone MS13, recognizes ~220 kDa BRCA1 in MCF-7 cells. It is validated for Western blotting, immunofluorescence, immunoprecipitation, and for paraffin sections.
Recognizes the ~220 kDa BRCA1 protein in MCF-7 cells. Does not react with blood group antigens or growth factor receptors such as EGFR. Sold under license of U.S. Patent 5,753,441 and 6,162,897.

免疫原

recombinant, human BRCA1
Epitope: within amino acids 1-304
Human

包装

100 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

应用

Immunoblotting (2 ?g/ml, chemiluminescence; see application references)

Immunofluorescence (see application references)

Immunoprecipitation (see application references)

Paraffin Sections (see application references)

警告

Toxicity: Standard Handling (A)

外形

In 0.05 M sodium phosphate buffer, 0.2% gelatin.

分析说明

Positive Control
MCF7 cells

其他说明

Scully, R., et al. 1996.Science272,123.
Gudas, J.M., et al. 1996.Cell Growth Differ.7,717.
Vaughn, J.P., et al. 1996.Cell Growth Differ.7,711.
Goldgar, D.E. and Reilly, P.R. 1995.Nat. Genet.11,113.
Merajver, S.D., et al. 1995.Clin. Can. Res.1,539.
Merajver, S.D., et al. 1995.Nat. Genet.9,439.
Struewing, J.P., et al. 1995.Nat. Genet.11,198.
Thompson, M.E., et al. 1995.Nat. Genet.9,444.
Futreal, P.A., et al. 1994.Science266,120.
Miki, Y., et al. 1994.Science266,66.
Antibody should be titrated for optimal results in individual systems.

法律信息

Sold under license of U.S. Patents 5,753,441 and 6,162,897.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified antibody
antibody product type	primary antibodies
克隆	MS13, monoclonal
形式	liquid
包含	≤0.1% sodium azide as preservative
species reactivity	human
manufacturer/tradename	Calbiochem?
储存条件	do not freeze
同位素/亚型	IgG1
运输	wet ice
储存温度	2-8℃

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable