Anti-BRCA2 (Ab-1) Mouse mAb (2B)

半岛bd体育手机客户端 说明

一般描述

Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with myeloma cells. Recognizes the ~460 kDa BRCA2 protein.
Anti-BRCA2 (Ab-1), mouse monoclonal, clone 2B, recognzies the ~460 kDa BRCA2 protein in cells expressing a wild type BRCA2 gene. It is validated for Western blotting and immunoprecipitation.
Recognzies the ~460 kDa BRCA2 protein in cells expressing a wild type BRCA2 gene. Sold under license of U.S. Patent 5,753,441 and 6,162,897.

免疫原

a recombinant protein consisting of amino acids 1651-1821 of human BRCA2 fused to GST
Human

包装

100 μg in Glass bottle
Please refer to vial label for lot-specific concentration.

应用

Immunoblotting (2 ?g/ml)

Immunoprecipitation (2 ?g)

警告

Toxicity: Standard Handling (A)

外形

In PBS.

重悬

Following initial thaw, aliquot and freeze (-20°C).

分析说明

Positive Control
MCF7 cells

其他说明

Andres, J.L., et al. 1998.Oncogene16,2229.
Chen, J., et al. 1998.Mol. Cell2,317.
Chen, P.L., et al. 1998.Proc. Natl. Acad. Sci. USA95,5287.
Marmorstein, L.Y., et al. 1998.Proc. Natl. Acad. Sci. USA95,13869.
Connor, F., et al. 1997.Nat. Genet.17,423.
Sharan, S.K., et al. 1997.Nature386,804.
Ludwig, T., et al. 1997.Genes Dev.11,1226.
Mizuta, R., et al. 1997.Proc. Natl. Acad. Sci. USA94,6927.
Rajan, J.V., et al. 1997.Dev. Biol.184,385.
Suzuki, A., et al. 1997.Genes Dev.11,1242.
Wong, A.K.C., et al. 1997.J. Biol. Chem.272,31941.
Cannon-Albright, L.A. and M.H. Skolnick. 1996.Semin. Oncol.23,1.
Goggins, M., et al. 1996.Cancer Res.56,5360.
Stratton, M.R. 1996.Hum. Mol. Genet.5,1515.
Tavtigian, S.V., et al. 1996.Nat. Genet.12,333.
Rajan, J.V., et al. 1996.Proc. Natl. Acad. Sci. USA93,13078.
Spillman, M.A. and A.M. Bowcock. 1996.Oncogene13,1639.
Vaughn, J.P., et al. 1996.Cancer Res.56,4590.
Wooster, R., et al. 1995.Nature378,789.
The CAPAN-1 cell line, which has 6174delT mutation in the single BRCA2 allele, expresses a truncated BRCA2 protein of 230 kDa that contains the Anti-BRCA2 epitope and retains RAD51 binding but has lost p53 binding. Full length BRCA2 protein will co-migrate on SDS-PAGE with the catalytic subunit of DNA PK which can be detected using Anti-DNA PK, Cat. No. PC127. Antibody should be titrated for optimal results in individual systems.

法律信息

Sold under license of U.S. Patents 5,837,492 and 6,124,104.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified antibody
antibody product type	primary antibodies
克隆	2B, monoclonal
形式	liquid
包含	≤0.1% sodium azide as preservative
species reactivity	human
manufacturer/tradename	Calbiochem?
储存条件	OK to freeze
同位素/亚型	IgG2b
运输	wet ice
储存温度	?20℃

安全信息

储存分类代码	11 - Combustible Solids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable