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位置: 首页> 品牌> Sigma-Aldrich> Anti-Aquaporin 4 Antibody, CT

Anti-Aquaporin 4 Antibody, CT

品牌
Sigma-Aldrich
货号
AB3594
规格纯度
Chemicon?, from rabbit
价格
6538.49 *本价格含增值税费
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数量
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半岛bd体育手机客户端 名称:
Anti-Aquaporin 4 Antibody, CT
Mercurial-Insensitive Water Channel
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special proteins "aquaporins". Aquaporins, are integral membrane proteins from a larger family of major intrinsic proteins (MIP) that form pores in the membrane of biological cells. Over the last decade, genes for several members of aquaporin family have been cloned, expressed, and their distribution studied in many tissues. The water-specific channel Aquaporin 4 is located in various tissues including the gastrointestinal tract, kidney and brain. It is an osmoreceptor which regulates body water balance and mediates water flow within the central nervous system. In CNS tissue, AQP4 localizes to astrocyte endfeet making it a useful structural marker.

特异性

Recognizes Aquaporin 4 (AQP4, WCH4, Mercurial-Insensitive Water Channel).
Reactivity with other species has not yet been tested. It is expected that the antibody may also react with bovine (71/75) and rabbit (64/75) due to sequence homology.

免疫原

Epitope: C-terminus
GST fusion protein with residues 249-323 of rat Aquaporin 4 (SwissProt accession number P47863).

应用

Research Sub Category
Ion & Transport Channels
Immunohistochemistry:
A previous lot of this antibody was used on rat brain sections.
Dilutions should be made using a carrier protein such as BSA (1-3%).

Western blotting:
1:500-1:1,000 using ECL on rat brain membranes.

Optimal working dilutions must be determined by the end user
Research Category
Neuroscience
Detect Aquaporin 4 using this Anti-Aquaporin 4 Antibody, C-terminus validated for use in IH & WB.

质量

Routinely evaluated by Western Blot on Mouse Brain lysates.
Western Blot Analysis: 1:500 dilution of this lot detected AQUAPORIN 4 on 10 μg of Mouse Brain lysates.

目标描述

38 kDa

外形

Purified rabbit polyclonal lyophilized from buffer containing PBS, pH 7.4, containing 1% BSA, and 0.05% sodium azide. Reconstitute with 200 ?L of sterile distilled water. Centrifuge antibody preparation before use (10,000 x g for 5 min).
ImmunoAffinity Purified
Format: Purified

储存及稳定性

Stable for up to 6 months at -20?C from date of receipt. After reconstitution stable at -20?C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

分析说明

Control
Included free of charge with the antibody is 120 ?g of control antigen (lyophilized powder). Reconstitute with 100 ?L of PBS. For positive control, in Western blot use 20 ng of protein per Minigel lane. For negative control, preincubate 3 ?g of purified peptide with 1 ?g of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss 32160702
NACRES NA.41

半岛bd体育手机客户端 性质

生物来源 rabbit
质量水平 100
抗体形式 affinity isolated antibody
antibody product type primary antibodies
克隆 polyclonal
species reactivity human, rat, mouse
manufacturer/tradename Chemicon?
technique(s) immunohistochemistry: suitable
western blot: suitable
NCBI登记号 NM_001650.4
UniProt登记号 P55087
运输 wet ice
Gene Information human ... AQP4(361)

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK WGK 2
闪点(F) Not applicable
闪点(C) Not applicable

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