Anti-Ankyrin_GMouse mAb (463)

半岛bd体育手机客户端 说明

一般描述

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with P3X63/Ag 8.653 mouse myeloma cells. Recognizes the ~190 kDa isoform of Ankyrin_G.
Anti-Ankyrin G, mouse monoclonal, clone 463, recognizes the ~190 kDa ankyrin G protein in brain extracts. It is validated for Western blotting, immunofluorescence, and frozen sections.
Recognizes the ~190 kDa ankyrin_Gprotein in brain extracts. Reacts with all spliced forms of ankyrin_G.

Antibody Target Gene Symbol: ANK3

Target Synonym: 2900054D09RIK, AI314020, AnkG, Ankyrin-3, ANKYRIN-G, FLJ45464, MGC14049

Entrez Gene Name: ankyrin 3, node of Ranvier (ankyrin G)

Hu Entrez ID: 288 (Related Antibodies: AP1034, PC523, PC522, AP1057)

Mu Entrez ID: 11735

Rat Entrez ID: 361833

免疫原

a synthetic peptide corresponding to amino acids from the spectrin-binding domain of human ankyrin_G
Human

包装

100 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

应用

Frozen Sections (1-2 g/ml)
Immunoblotting (2-5 g/ml)
Immunofluorescence (1-2 g/ml)

警告

Toxicity: Standard Handling (A)

外形

In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.

分析说明

Positive Control
Mouse or rat brain extract or unmyelinated axons

其他说明

Ankyrin_Gis a good immunohistochemical marker for axon initial segments, nodes of Ranvier, and epithelial cell-cell contact sites. Antibody should be titrated for optimal results in individual systems.
Davis, J.Q., et al. 1995.Mol. Biol. Cell6, 177a, supplement.
Kunimoto, M., et al. 1995.FEBS Lett.357, 217.
Lambert, S., et al. 1995.Mol. Biol. Cell6, 98a supplement.
Bennett, V. and Gilligan, D.M. 1993.Ann. Rev. Cell Biol.9, 27.
Kunimoto, M., et al. 1991.J. Cell Biol.115, 1319.
Otto, E., et al. 1991.J. Cell Biol.114, 241.
Davis, J., and Bennett, V. 1984.J. Biol. Chem.259, 13550.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified antibody
antibody product type	primary antibodies
克隆	463, monoclonal
测定	≥95% (SDS-PAGE)
形式	liquid
包含	≤0.1% sodium azide as preservative
species reactivity	mouse, canine, rat, human
manufacturer/tradename	Calbiochem?
储存条件	do not freeze
同位素/亚型	IgG1
运输	wet ice
储存温度	2-8℃

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	nwg
闪点(F)	Not applicable
闪点(C)	Not applicable