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位置: 首页> 品牌> Sigma-Aldrich> Anti-Adenylate Cyclase 5 Antibody, clone 19D5.C1

Anti-Adenylate Cyclase 5 Antibody, clone 19D5.C1

品牌
Sigma-Aldrich
货号
MABS2049
规格纯度
clone 19D5.C1, from mouse
价格
1415.25 *本价格含增值税费
促销
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数量
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半岛bd体育手机客户端 名称:
Anti-Adenylate Cyclase 5 Antibody, clone 19D5.C1
Adenylate cyclase type 5, EC: 4.6.1.1, ATP pyrophosphate-lyase 5, Adenylate cyclase type V, Adenylyl cyclase 5
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Adenylate cyclase type 5 (P84309; also known as EC: 4.6.1.1, ATP pyrophosphate-lyase 5, Adenylate cyclase type V, Adenylyl cyclase 5, AC5) is encoded by the Adcy5 gene (Gene ID: 224129) in murine species. Adenylate cyclase catalyzes the formation of cyclic AMP in response to G-protein signaling. It regulates the increase of free cytosolic calcium in response to increased blood glucose levels and contributes to the regulation of calcium-dependent insulin secretion. Adenylyl cyclases are typically made up of two clusters of six transmembrane domains separating two intracellular, overlapping catalytic domains. The amino acid sequence of each cytoplasmic domain that contains the nucleotide (ATP)-binding site, is well conserved among various subtypes. Thus far 9 different hormone-sensitive, membrane-bound AC isoforms have been identified. Adenylyl Cyclase 5 deficiency is shown to protect against diet-induced obesity and insulin resistance in mice and extends their life span. Adenylate cyclase 5 knockout mice display signs of reduced stress and protection against the stress of chronic pressure overload on heart. Adenylate cyclase 5 activity is stimulated by forskolin and is inhibited by adenosine and ATP analogs. (Ref.: Ho, D., et al. (2015). Diabetes. 64(7); 2636-2645. Vatner, SF., et al. (2015). Oxid. Med. Cell. Longev. 2015:250310).

特异性

Clone 19D5.C1 detects Adnylate Cyclase 5 in murine, rat, and procine cells. It targets an epitope within 20 amino acids from the cytoplasmic domain.

免疫原

Epitope: cytoplasmic domain
KLH-conjugated linear peptide corresponding to 20 amino acids from the cytoplasmic domain of murine adenylate cyclase V.

应用

Anti-Adenylate Cyclase 5, clone 19D5.C1, Cat. No. MABS2049, is a mouse monoclonal antibody that detects adenylate cyclase 5 and has been tested for use in ELISA and Western Blotting.
Western Blotting Analysis: A representative lot detected Adenylyl Cyclase 5 in Western Blotting applications (Hu, C.L., et. al. (2009). Am J Physiol Heart Circ Physiol. 297(5):H1776-82; De Lorenzo, M.S., et. al. (2014). Aging Cell. 13(1):102-10).

ELISA Analysis: A representative lot detected Adenylyl Cyclase 5 in ELISA applications (Hu, C.L., et. al. (2009). Am J Physiol Heart Circ Physiol. 297(5):H1776-82).

Research Category
Signaling

质量

Evaluated by Western Blotting in mouse brain membrane preparations.

Western Blotting Analysis: 1 ?g/mL of this antibody detected Adenylate Cyclase 5 in mouse brain membrane preparations.

目标描述

~150 kDa observed; 139.12 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

外形

Protein G purified
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Format: Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss 32160702
NACRES NA.41

半岛bd体育手机客户端 性质

生物来源 mouse
抗体形式 purified antibody
antibody product type primary antibodies
克隆 19D5.C1, monoclonal
species reactivity rat, porcine, mouse
species reactivity (predicted by homology) human (based on 100% sequence homology)
包装 antibody small pack of 25 μg
technique(s) ELISA: suitable
western blot: suitable
同位素/亚型 IgG1κ
NCBI登记号 NP_001012783.3
UniProt登记号 P84309

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK WGK 1
闪点(F) Not applicable
闪点(C) Not applicable

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