半岛bd体育手机客户端 说明
一般描述
Recognizes α-actin in smooth muscle cells of blood vessels and parenchymal tissue of the intestine, testis, and ovary. Does not recognize actin from fibroblasts (β- and γ-cytoplasmic), striated muscle (α-sarcomeric), or myocardium (α-myocardial).
免疫原
Human Smooth Muscle
a synthetic peptide corresponding to amino acids 1-10 of human smooth muscle α-actin.
包装
500 μL in Plastic ampoule
Please refer to vial label for lot-specific concentration.
应用
Frozen Sections (1:50-1:75)
Immunoblotting (see comments)
Paraffin Sections (1:50-1:75)
警告
Toxicity: Standard Handling (A)
外形
In 150 mM NaCl, 20 mM Tris-borate, BHC-6 carrier polysaccharides, pH 7.5.
分析说明
Positive Control
Smooth muscle cells of blood vessels and parenchymal tissue of intestine, testis, or ovary
Negative Control
Fibroblasts, striated muscle, or myocardium
其他说明
Rannov-Jennsen, L., et al. 1990.Lab Invest.63,532.
Skalli, O., et al. 1989.J. Histochem. Cytochem37,315.
Skalli, O., et al. 1986.J. Cell. Biol.103,2787.
Does not recognize actin from fibroblasts (β- and γ-cytoplasmic), striated muscle (α-sarcomeric), or myocardium (α-myocardial).Prolonged fixation in buffered formalin destroys the epitope. The antibody is specific for the α-smooth muscle isoform of actin. This antibody is also reported to work for immunoblotting. Variables associated with assay conditions will dictate the proper working dilution.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
半岛bd体育手机客户端 性质
质量水平 | 100 |
生物来源 | mouse |
antibody product type | primary antibodies |
克隆 | 1A4, monoclonal |
形式 | liquid |
包含 | ≤0.1% sodium azide as preservative |
species reactivity | human, rabbit, baboon |
manufacturer/tradename | Calbiochem? |
储存条件 | do not freeze |
同位素/亚型 | IgG2a |
运输 | wet ice |
储存温度 | 2-8℃ |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 2 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich