半岛bd体育手机客户端 说明
一般描述
Recombinant,Bacillus anthracisanthrax protective antigen expressed in a specialized strain ofB. anthracis. One of the three protein components of Anthrax toxin, protective antigen (PA) is the central moiety that mediates the entry of lethal factor and edema factor into the target cell. PA binds to the cell surface via a type I membrane protein with a von Willebrand factor A domain called anthrax toxin receptor. After proteolytic activation to a 63 kDa fragment, PA self-associates to form a heptameric cation-selective, voltage-gated membrane channel.
Recombinant,Bacillus anthracisanthrax protective antigen expressed in a special strain ofB. anthracis. One of three protein components of anthrax toxin produced by the pathogenic bacterium,Bacillus anthracis. Central moiety that mediates entry of lethal factor (LF) and edema factor (EF) into the target cell. PA83 binds to the cell surface via a type I membrane receptor with a von Willebrand factor A domain, known as anthrax toxin receptor. PA83 is proteolytically activated in vivo by a furin-like protease to produce a 63 kDa protein (PA63). PA63 self-associates to form a heptameric, cation-selective, voltage-gated, membrane channel that subsequently binds to LF and/or EF. Cleavage of the 20 kDa fragment from PA83 is necessary for binding of protective antigen to LF and EF.
包装
100 μg in Plastic ampoule
yes
生化/生理作用
Product does not compete with ATP.
Reversible: no
Cell permeable: no
Primary Target
Type I membrane protein with a von Willebrand factor A domain called anthrax toxin receptor
警告
Toxicity: Standard Handling (A)
外形
Lyophilized from 50 mM NaCl, 5 mM HEPES, pH 7.5.
重悬
Following reconstitution aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 6 months at -20°C.
Reconstitute in 100 ?l sterile, distilled H?O to yield a final concentration of 1 mg/ml.
分析说明
Single major band by SDS-PAGE. Several smaller minor components are apparent that may represent cleavage fragments of the PA.
其他说明
Mogridge, J., et al. 2002.Biochemistry41,1079.
Nassi, S., et al. 2002.Biochemistry41,1445.
Ahuja, N., et al. 2001.Biochem. Biophys. Res. Commun.286,6.
Bradley, K.A., et al. 2001.Nature414,225.
Brossier, F., and Mock, M. 2001.Toxicon39,1747.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
半岛bd体育手机客户端 性质
| 质量水平 | 100 |
| 形式 | lyophilized |
| manufacturer/tradename | Calbiochem? |
| 储存条件 | OK to freeze |
| 运输 | ambient |
| 储存温度 | 2-8℃ |
安全信息
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
