半岛bd体育手机客户端 说明
一般描述
Recombinant,Clostridium perfringensα2-3,6-Neuraminidase expressed inE. coli. Catalyzes the hydrolysis of non-reducing terminal α2,3 and α2,6 unbranched sialic acid residues from complex carbohydrates and glycoproteins. This enzyme does not exhibit activity on α2,8 or branched sialic acids.
Note: 1 mU = 1 milliunit.
包装
500 miu in Plastic ampoule
应用
Recommended reaction buffer: 50 mM sodium phosphate buffer, pH 6.0
警告
Toxicity: Harmful (C)
单位定义
One unit is defined as the amount of enzyme that will catalyze the release of 1 ?mol of methylumbelliferone from 2?-(4-Methylumbelliferyl)-α-D-N-acetylneuraminic Acid (Cat. No. 474495) per min at 37°C, pH 5.0.
外形
In 25 mM NaCl, 20 mM Tris-HCl, pH 7.5.
其他说明
Prime, S., et al. 1996.J. Chromatogr. A.720,263.
Dwek, R.A., et al. 1993.Annu. Rev. Biochem.62,65.
Ohta, Y., et al. 1989.J. Biochem (Tokyo)106,1086.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Emprove is a registered trademark of Merck KGaA, Darmstadt, Germany
基本信息
酶学委员会(EC)编号 | 3.2.1.18 ( BRENDA | IUBMB ) |
半岛bd体育手机客户端 性质
质量水平 | 100 |
重组 | expressed inE. coli |
半岛bd体育手机客户端 线 | EMPROVE?EXPERT |
形式 | liquid |
specific activity | ≥10 units/mL ≥250 units/mg protein |
manufacturer/tradename | Calbiochem? |
储存条件 | do not freeze |
异质活性 | N-acetylglucosaminidase, α-fucosidase, α- and β-galactosidase, α- and β-mannosidase, proteases, none detected |
运输 | wet ice |
储存温度 | 2-8℃ |
安全信息
象形图 | |
警示用语: | Warning |
危险声明 | H290 - H315 - H319 - H335 |
预防措施声明 | P234 - P261 - P264 - P271 - P302 + P352 - P305 + P351 + P338 |
危险分类 | Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3 |
靶器官 | Respiratory system |
储存分类代码 | 8B - Non-combustible, corrosive hazardous materials |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich