α1,6-Fucosidase,Chryseobacterium meningosepticum, Recombinant,E. coli

半岛bd体育手机客户端 说明

一般描述

Recombinant,Chryseobacterium meningosepticumα1,6-fucosidase expressed inE. coli. This enzyme fromChryseobacterium(formerlyFlavobacterium) meningosepticumcatalyzes the hydrolysis of branched non-reducing terminal α1,6-linked fucose residues from the N-acetylglucosamine core of N-Linked oligosaccharides. The reducing terminus of the oligosaccharide must be covalently bonded to a reporter molecule, such as aminonaphthalene disulfonic acid (ANDSA), aminonaphthalene trisulfonate (ANTS), and 2-aminoanthanilic acid (2-AA). The one exception is that a terminal unbranched α1,3-fucose is cleaved in the absence of any reporter molecule. Does not cleave fucose from glycoproteins.
Note: 1 mU = 1 milliunit.
Recombinant,Chryseobacterium meningosepticumα1,6-fucosidase expressed inE. coli. This enzyme fromChryseobacterium(formerlyFlavobacterium meningosepticum) catalyses the hydrolysis of branched non-reducing terminal α1,6-linked fucose residues from the N-acetylglucosamine core of N-linked oligosaccharides. The reducing terminus of the oligosaccharide must be covalently bonded to a reporter molecule, such as aminonaphthalene disulfonic acid (ANDSA), aminonaphthalene trisulfonate (ANTS), and 2-aminoanthanilic acid (2-AA). The one exception is that a terminal unbranched α1,3-fucose is cleaved in the absence of any reporter molecule. Does not cleave fucose from glycoproteins.

包装

50 miu in Plastic ampoule

警告

Toxicity: Standard Handling (A)

单位定义

One unit is defined as the amount of enzyme that will release 1.0 ?mol methylumbelliferone from 4-methylumbelliferyl-α-L-fucoside per min at 37°C, pH 5.0.

外形

In 25 mM NaCl, 20 mM Tris-HCl, pH 7.5.

其他说明

Zheng, Q., et al. 2002.J. Biol. Chem.277,39823.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

基本信息

酶学委员会(EC)编号

3.2.1.51 ( BRENDA | IUBMB )

半岛bd体育手机客户端 性质

质量水平	100
重组	expressed inE. coli
形式	liquid
specific activity	≥1.0 units/mL ≥1.5 units/mg protein
manufacturer/tradename	Calbiochem?
储存条件	do not freeze
异质活性	Proteases, none detected
运输	wet ice
储存温度	2-8℃

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable