半岛bd体育手机客户端 说明
一般描述
KiCqStart SYBR Green qPCR ReadyMix is a 2X concentrated, ready-to-use reaction cocktail that contains all components, except primers and template, for real-time quantitative PCR (qPCR) This unique combination of proprietary buffer, stabilizers, and Hot-Start Taq DNA polymerase delivers maximum PCR efficiency, sensitivity, specificity and robust fluorescent signal using fast, or conventional, cycling protocols with SYBR Green qPCR.
Highly specific amplification is crucial to successful qPCR with SYBR Green I dye technology because this dye binds to and detects any dsDNA generated during amplification. Hot-Start Taq DNA polymerase is antibody mediated to be inactive prior to the initial PCR denaturation step. The optimized formulation includes SYBR Green I dye, an antibody-mediated Hot-Start Taq DNA polymerase, dNPTs, MgCl2and proprietary buffers and stabilizers.
应用
Instrument Compatibility:
Different real-time PCR systems employ different strategies for the normalization of fluorescent signals and correction of well-to-well optical variations. It is critical to match the appropriate qPCR reagent to your specific instrument. KiCqStart SYBR Green qPCR ReadyMix, iQ contains fluorescein for experimental plate well factor collection on iCycler iQ real-time detection systems or the MyiQ detection system. Compatible instruments include:
特点和优势
组分
2X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP), KiCqStart Taq DNA Polymerase, SYBR Green dye, 20 nM fluorescein, and stabilizers
packaging:
250 reactions* = 2 X 1.25 mL tubes
1250 reactions* = 10 X 1.25 mL tubes
5000 reactions* = 1 X 50 mL tube
*number of reactions based on a 20uL volume
其他说明
Storage Conditions:
KiCqStart SYBR Green qPCR ReadyMix is stable for 1 year when stored in a constant temperature freezer at -20℃, protected from light. For convenience, it may be stored unfrozen at +2 to +8℃ for up to 6 months. After thawing, mix thoroughly before using. Repeated freezing and thawing of the product is not recommended. However, the product demonstrated no loss of performance after 20 freeze-thaw cycles or 2 months at +20℃.
法律信息
KiCqStart is a registered trademark of Qiagen Beverly Inc.
MyiQ is a trademark of Bio-Rad Laboratories, Inc.
ReadyMix is a trademark of sigma-aldrich Co. LLC
SYBR is a registered trademark of Life Technologies
iCycler iQ is a registered trademark of Bio-Rad Laboratories, Inc.
iQ is a trademark of Bio-Rad Laboratories, Inc.
基本信息
NACRES | NA.55 |
半岛bd体育手机客户端 性质
形式 | liquid |
用途 | sufficient for 1250 reactions sufficient for 250 reactions sufficient for 5000 reactions |
特点 | dNTPs included hotstart |
储存条件 | protect from light |
technique(s) | qPCR: suitable |
颜色 | colorless |
input | purified DNA |
相容性 | for use with Bio-Rad MyiQ for use with Bio-Rad iCycler iQ for use with Bio-Rad iQ 5 |
detection method | SYBR?Green |
运输 | dry ice |
储存温度 | ?20℃ |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 3 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich