Anti-v-Src (Ab-1) Mouse mAb (327)

半岛bd体育手机客户端 说明

一般描述

Recognizes the ~60 kDa v-Src and c-Src proteins in NIH/3T3 and HT1080 cells. May appear as a doublet due to phosphorylation.
Purified mouse monoclonal generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with P3X63 Ag8.653 myeloma cells (see application references). Recognizes the ~60 kDa Src protein.
This Anti-v-Src (Ab-1) Mouse mAb (327) is validated for use in Immunoblotting, Immunoprecipitation, Frozen Sections, Immunofluorescence, Paraffin Sections for the detection of v-Src (Ab-1).

免疫原

purified v-Src
Epitope: within the SH3 domain of v-Src and c-Src

包装

100 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

应用

Immunoblotting (2.5 ?g/ml, see application references)

Immunoprecipitation (1 ?g antibody or use Cat. No. OP07A; see application references)

Frozen Sections (not recommended)

Immunofluorescence (not recommended)

Paraffin Sections (not recommended)

警告

Toxicity: Standard Handling (A)

外形

In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.4.

分析说明

Positive Control
NIH/3T3 cells

其他说明

pp60^srcmay appear as a doublet due to phosphorylation. This antibody can be used to precipitate activesrckinase (see application references). The agarose conjugate, Cat. No. OP07A, is available separately for immunoprecipitation. Antibody should be titrated for optimal results in individual systems.
Courtneidge, S.A. and Heber, A. 1987.Cell50, 1031.
Cartwright, C.A., et al. 1985.Mol. Cell. Biol.5,, 2647.
Courtneidge, S.A. 1985.EMBO J.4, 1471.
Whitman, M., et al. 1985.Nature315, 239
Bolen, J.B., et al. 1984.Cell38, 767.
Hunter, T. and Sefton, B. 1980.Proc. Natl. Acad. Sci.77, 1311.
Collett, M.S. et al. 1979.Proc. Natl. Acad. Sci.76, 3159.
Collett, M.S. and Erikson, R.L. 1978.Proc. Natl. Acad. Sci.75, 2021.
Collett, M.S., et al. 1978.Cell15, 1363.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified antibody
antibody product type	primary antibodies
克隆	327, monoclonal
形式	liquid
包含	≤0.1% sodium azide as preservative
species reactivity	human, mouse, rat
manufacturer/tradename	Calbiochem?
储存条件	do not freeze
同位素/亚型	IgG1
运输	wet ice
储存温度	2-8℃

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	nwg
闪点(F)	Not applicable
闪点(C)	Not applicable