Anti-c-Abl (Ab-3) Mouse mAb (24-21)

半岛bd体育手机客户端 说明

一般描述

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with p.3U1 mouse myeloma cells (see application references). Recognizes the v-Abl, the ~145-150 kDa c-Abl, the ~210 kDa Bcr-Abl (CML), and the ~190 kDa Bcr-Abl (ALL) proteins.
Anti-c-Abl (Ab-3), mouse monoclonal, clone 24-21, recognizes the ~145 kDa human and ~150 kDa mouse c-Abl. It is validated for Western blotting, immunofluorescence, and immunoprecipitation.
Recognizes the ~145 kDa human c-Abl, the ~150 kDa mouse c-Abl, v-Abl, and the Bcr/Abl translocation products in ALL (~190 kDa) and CML (~210 kDa). Does not inhibit protein tyrosine kinase activity.

Antibody Target Gene Symbol: ABL1

Target Synonym: ABL, AI325092, bcr/abl, C-ABL, C-ABL 1B, CABL1, E430008G22Rik, JTK7, MGC117749, p145Abl, p150, v-abl

Entrez Gene Name: c-abl oncogene 1, receptor tyrosine kinase

Hu Entrez ID: 25 (Related Antibodies: PK1006PK1013OP19)

Mu Entrez ID: 11350

Rat Entrez ID: 311860

免疫原

a recombinant protein consisting of the carboxyl region of v-abl protein fused to TrpE

包装

100 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

应用

Immunoblotting (1-5 ?g/ml)

Immunofluorescence (see application references)

Immunoprecipitation (1-2 ?g/sample)

Neutralization Studies (not recommended)

警告

Toxicity: Standard Handling (A)

外形

In 0.05 M sodium phosphate buffer, 0.2% gelatin, pH 7.5.

分析说明

Positive Control
K562 (humanbcr/abl), HL-60 (normalabl), and ANN-1 (murineabl) cells

其他说明

Does not inhibit protein tyrosine kinase activity. Detects human c-Abl (145 kDa), mouse c-Abl (150 kDa), v-Abl, and the Bcr/Abl translocation products in ALL (~210 kDa) and CML (~190 kDa). HL-60 and NIH3T3 cells contain normal, non-rearranged protein. Antibody should be titrated for optimal results in individual sample types.
Wiedemann, L.M., et al. 1988.Blood71,349.
Stam, K., et al. 1985.N. Engl. J. Med.313,1429.
Konopka, J.B., et al. 1984.Cell37,1035.
Prywes, R., et al. 1983.Cell34,569.
de Klein, A., et al. 1982.Nature300,765.
Reynolds, F.H., Jr., et al. 1980.J. Virol.36,374.
Reynolds, F.H., Jr., et al. 1978.Proc. Natl. Acad. Sci. USA75,3974.
Witte, O.N., et al. 1978.Proc. Natl. Acad. Sci. USA75,2488.
Abelson, H.T. and Rabstein, L.S. 1970.Cancer Res.30,2213.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified antibody
antibody product type	primary antibodies
克隆	24-21, monoclonal
形式	liquid
包含	≤0.1% sodium azide as preservative
species reactivity	human, mouse
manufacturer/tradename	Calbiochem?
储存条件	do not freeze
同位素/亚型	IgG1
运输	wet ice
储存温度	2-8℃

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	nwg
闪点(F)	Not applicable
闪点(C)	Not applicable