半岛bd体育手机客户端 说明
一般描述
Each Universal ProbeLibrary probe is a single hydrolysis probe that recognizes sequences from one or more of the following organisms: humans, chimpanzees and other primates, mice, rats,C. elegans, Drosophila, Arabidopsis.
Short hydrolysis probes for quantification of gene expression levels by real-time PCR.
应用
Use the Universal ProbeLibrary Probes to obtain rapid, flexible quantification of expression levels for virtually any gene from the human, mouse, rat, chimpanzee,C. elegans,Drosophila,orArabidopsistranscriptomes in the NCBI Reference Sequence Database. Each probe can be used with standard reagents in real-time PCR assays:
特点和优势
Each of the Universal ProbeLibrary probes:
组分
Two vials, each containing 125 μL of one probe solution [10 μM solution of a single probe, 5′ labeled with fluorescein (FAM) and 3′ labeled with a dark quencher dye].
质量
Function test: All Universal ProbeLibrary probes are tested for performance in real-time PCR using primers and control templates from the Universal ProbeLibrary Control Set. Each probe must pass this test.
Purity: Each probe is analyzed by anion-exchange HPLC.
Composition: Each probe is analyzed by MALDI-MS.
其他说明
Universal ProbeLibrary System User Statements and Application
仅用于生命科学研究。不可用于诊断。
Probe chemistry: Universal ProbeLibrary probes use a unique nucleotide chemistry called LNA (Locked Nucleic Acid), which allows very short (8-9 bases) oligonucleotides to be effective hybridization probes in real-time PCR assays. LNA allows the melting temperature of the short probes to be unusually high. Thus, they are fully compatible with commonly used PCR conditions and the hydrolysis-probe detection format. These probes are even sensitive enough to detect single-base mismatches.
Assay design: The free online ProbeFinder software, available at theUniversal ProbeLibrary Assay Design Center, designs one or more intron-spanning assays for a target gene, based on specific information (i.e., gene name, accession number, or sequence) that you submit. For each assay it designs, the software will specify a set of specific primers plus a probe from the Universal ProbeLibrary that will give the best results. The combination of primers and probe will provide specific amplification and detection of your target sequence in a standard real-time PCR assay.
Labeling: pre-labeled with a reporter fluorophore (FAM) and a dark quencher dye.
Instrumentation required: standard real-time PCR instrumentation.
Notes: In addition to being available individually, the Universal ProbeLibrary probes are available in sets (90 probes/set), each of which covers virtually all the transcripts from a single organism. Together, the sets cover approximately 99% of the human, primate, mouse, rat,C. elegans,Drosophila, andArabidopsisgene transcripts listed in the NCBI Reference Sequence Database.
For more information, see the listings for these organism-specific sets.
Universal ProbeLibrary System Performance Data
Universal ProbeLibrary System Technology
法律信息
LightCycler is a registered trademark of Roche
TaqMan is a registered trademark of Roche Molecular Systems, Inc.
半岛bd体育手机客户端 性质
质量水平 | 100 |
manufacturer/tradename | Roche |
包装 | pkg of Probe #41 (04688007001) pkg of Probe #42 (04688015001) pkg of Probe #43 (04688031001) pkg of Probe #44 (04688040001) pkg of Probe #45 (04688058001) pkg of Probe #46 (04688066001) pkg of Probe #47 (04688074001) pkg of Probe #48 (04688082001) pkg of Probe #49 (04688104001) pkg of Probe #50 (04688112001) |
Sigma-Aldrich