半岛bd体育手机客户端 说明
应用
Useful for kinase assay and Western blot.
生化/生理作用
Recombinant human YSK4 (MAP3K19) (941-end) was expressed byBaculovirusin Sf9 insect cells using an N-terminal GST tag. YSK4 (MAP3K19, RCK) belongs to STE Ser/Thr protein kinase family and STE20 subfamily. The YSK4 gene is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, and rat(1). The important paralog is MAP3K1 gene. The RCK ligand-binding domains form an octameric gating ring(2)in the large-conductance Ca2+-activated K+channels(3). The YSK4 kinase function has not been made clearly yet.
重悬
Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.
储存及稳定性
Store in aliquots at -70℃. Avoid freeze/thaw cycles.
法律信息
PRECISIO is a registered trademark of sigma-aldrich Co. LLC
半岛bd体育手机客户端 性质
| 重组 | expressed in baculovirus infectedSf9 cells |
| 测定 | 70-95% (SDS-PAGE) |
| 形式 | frozen liquid |
| specific activity | 30-45 nmol/min·mg (radiometric) |
| 分子量 | ~83 kDa |
| 浓度 | 0.05 μg/μL |
| 登记号 | NM_025052 |
| 运输 | dry ice |
| 储存温度 | ?70℃ |
| Gene Information | human ... YSK4(80122) |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
