Vimentin, His tagged human

半岛bd体育手机客户端 说明

一般描述

Vimentin belongs to the intermediate filament family of proteins. It is a key structural component of eukaryotic cells and is associated with lymphocytes and neutrophils. Vimentin exists as a tripartite domain structure. It is expressed in mesenchymal cells. The vimentin gene is mapped to human chromosome 10p13.

应用

Vimentin, His tagged human has been used in the glass surface immobilization in anti-vimentin binding in force measurement by atomic force microscopy (AFM).

生化/生理作用

Vimentin (VIM) stabilizes cytoskeletal interactions and is crucial for the placement of organelles in the cytosol. It modulates apoptosis in lymphocytes and is involved in the pathogenesis of inflammatory diseases VIM may serve as a potential marker for sepsis diagnosis. It is also essential for lens integrity maintenance.
Vimentin is a member of intermediate filament family of proteins and is an important structural feature of eukaryotic cells. Along with microtubules and actin microfilaments, Vimentin make up the cytoskeleton component of cells. Studies have shown that Vimentin is attached to the nucleus, endoplasmic reticulum and mitochondria, either laterally or terminally. Vimentin plays a significant role in supporting and anchoring the position of the organelles in the cytosol. Thus, Vimentin plays a key role in maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions

外形

Supplied in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.

制备说明

after opening, aliquot into smaller quantities and store at -70 ℃. Avoid repeating handling and multiple freeze/thaw cycles

基本信息

NACRES

NA.32

半岛bd体育手机客户端 性质

生物来源	human
重组	expressed inE. coli
测定	≥90% (SDS-PAGE)
形式	buffered aqueous glycerol solution
分子量	~60 kDa
NCBI登记号	NM_003380
application(s)	cell analysis
运输	dry ice
储存温度	?70℃
Gene Information	human ... VIM(7431)

安全信息

象形图
警示用语：	Danger
危险声明	H315 - H319 - H360D
预防措施声明	P202 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313
危险分类	Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
储存分类代码	6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
WGK	WGK 1