半岛bd体育手机客户端 说明
一般描述
Thymic stromal lymphopoietin (TSLP) is a hemopoietic cytokine. It is part of the interleukin-2 (IL-2) cytokine family. It is mainly expressed in the heart, liver and prostate. TSLP is related in its biological activities to interleukin-7 (IL-7) and binds with the heterodimeric receptor complex consisting of interleukin-7 receptor α chain & the TSLP receptor. Recombinant human TSLP produced inEscherichia coliis a single, non-glycosylated polypeptide chain containing 132 amino acids and having a molecular mass of 15kDa.
应用
TSLP human has been used in immunohistochemistry.
生化/生理作用
Thymic stromal lymphopoietin (TSLP) signals throughout a heterodimeric receptor complex composed of the thymic stromal lymphopoietin receptor and the interleukin-7 (IL-7) receptor a chain. TSLP impacts myeloid cells and thus induces the discharge of T cell-attracting chemokines from monocytes and increases the growth of CD11c+dendritic cells. Similar to IL-7, TSLP enhances phosphorylation of signal transducer and activator of transcription 3 and 5 (STAT3 and STAT5), though uses kinases excluding Janus kinases (JAKs) for its activation. TSLP induces the release of T cell-attracting chemokines such as thymus and activation-regulated chemokine (TARC) and macrophage-derived chemokine (MDC) from monocytes and triggers CD11c+dendritic cells. TSLP activated dendritic cells primes naive T cells to manufacture pro-allergic cytokines such as interleukin-4, interleukin-5, interleukin-13 and tumor necrosis factor-α (TNF-α), whereas down-regulating interleukin-10 and interferon-γ (IFN-γ) play a role in the initiation of allergic inflammation.
外形
Lyophilized from a concentrated solution (1 mg/ml) containing 130 mM NaCl, and 20 mM sodium phosphate, pH 7.4.
重悬
Reconstitute in sterile dH2O to a concentration of 0.1 -1 mg/mL and let the lyophilized pellet dissolve completely. This solution can then be diluted into other aqueous buffers.
Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.
基本信息
| NACRES | NA.32 |
半岛bd体育手机客户端 性质
| 生物来源 | human |
| 重组 | expressed inE. coli |
| 测定 | ≥97% (HPLC) ≥97% (SDS-PAGE) |
| 形式 | lyophilized |
| 效能 | <0.3 ng/mL ED50 |
| 分子量 | ~15.0 kDa |
| 包装 | pkg of 20 μg |
| 储存条件 | avoid repeated freeze/thaw cycles |
| 杂质 | endotoxin, tested |
| NCBI登记号 | Q969D9 |
| 运输 | wet ice |
| 储存温度 | ?20℃ |
| Gene Information | human ... TSLP(85480) |
安全信息
| 储存分类代码 | 13 - Non Combustible Solids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
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