半岛bd体育手机客户端 说明

应用

TBE running buffer is the most commonly used buffer for DNA and RNA polyacrylamide gel electrophoresis. TBE is used with non-denaturing or denaturing (7 M urea) gels. It is also routinely used for DNA automated sequencing gel. Tris-Borate-EDTA buffer has been used for pulsed-field gel electrophoresis (PFGE). Applied voltages of less than 5 V/cm are recommended for maximum resolution.
TBE电泳液是DNA和RNA聚丙烯酰胺凝胶电泳最常用的缓冲液。TBE用于非变性或变性(7M 尿素)凝胶。 它通常也用于DNA自测序凝胶。 TBE可以用于琼脂糖凝胶，但是不推荐用于回收核酸的制备胶。
将TBE储存浓缩溶液稀释到1×TBE电泳缓冲液，生成的缓冲液含有89mM Tris-硼酸和2mM EDTA，pH8.3。 5X或10X储存液也可以加入丙烯酰胺/甲叉双丙烯酰胺储存液，用来制成PAGE胶。 为了达到最大的分辨率，推荐电压小于5V/cm(电极单元间距离)。

包装

Foil pouches

制备说明

采用经生物技术性能认证的 Trizma 碱(半岛bd体育手机客户端 代码 T6066)、分子生物学试剂硼酸(半岛bd体育手机客户端 代码 B6768)和 EDTA 二钠盐(半岛bd体育手机客户端 代码 E5134)制备。

重悬

Contents of one pouch, when dissolved in 500mL of distilled or deionized water, will yield a 1X solution containing 89mM Tris-Borate, 2mM EDTA, pH 8.3 at 25 ℃. Certified to be DNAse, RNAse, Protease, and Nickase free. Certified for use in electrophoresis. Recommended temperature for dissolution 5 ℃ to 40 ℃.

储存及稳定性

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

法律信息

pHast Pack is a trademark of sigma-aldrich Co. LLC

基本信息

MDL编号	MFCD00236358
NACRES	NA.21

半岛bd体育手机客户端 性质

半岛bd体育手机客户端 线	pHast Pack?
形式	powder
pH值(酸碱度)	8.3
SMILES string	OB(O)O.NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
InChI	1S/C10H16N2O8.C4H11NO3.BH3O3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;2-1(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;2-4H
InChI key	OSBLTNPMIGYQGY-UHFFFAOYSA-N

安全信息

象形图
警示用语：	Danger
危险声明	H360FD
预防措施声明	P201 - P202 - P280 - P308 + P313 - P405 - P501
危险分类	Repr. 1B
储存分类代码	6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable