TRAIL, Soluble mouse

半岛bd体育手机客户端 说明

一般描述

Tumor Necrosis Factor (TNF)-Related Apoptosis-Inducing Ligand (TRAIL) is a type II transmembrane protein and belongs to the TNF superfamily of ligands. Trimerisation of TRAIL around a centrally positioned Zn atom is needed for maintaining its stability, solubility and biological activity as a death ligand. TRAIL exists in two forms, soluble and membrane bound.

生化/生理作用

Tumor Necrosis Factor (TNF)-Related Apoptosis-Inducing Ligand (TRAIL) activates the extrinsic apoptotic pathway by binding death receptors (DR) 4 and 5. In addition to DR 4 and 5, TRAIL activates several other receptors such as decoy receptor (DcR)1, DcR2 and a soluble receptor called osteoprotegerin. TRAIL plays a vital role in the inhibition of autoimmune inflammation and cell cycle progression. TRAIL selectively kills tumor cells without damaging normal tissues and therefore, it might act as a tumor-selective anticancer agent. TRAIL-induced apoptosis is involved in various cellular processes including activation-induced cell death, homeostasis, intrathymic negative selection, autoimmunity suppression, and immune surveillance. Repeated dosage of recombinant soluble TRAIL triggers apoptosis, inhibits tumor progression, and increases viability of mice suffering from cancer.

外形

Solution in 20 mM HEPES, pH 7.4, 300 mM NaCl, 0.01% Tween 20, 1% sucrose, 1 mM DTT.

其他说明

The extracellular domain of mouse TRAIL (aa 99-291) is fused at the N-terminus to a His-tag and a linker peptide. The active multimeric conformation is stabilized by an inserted mutation allowing an additional CC-bridge.

基本信息

NACRES

NA.32

半岛bd体育手机客户端 性质

生物来源	mouse
重组	expressed inE. coli
标签	His tagged
测定	>95% (SDS-PAGE)
形式	liquid
分子量	~24 kDa
包装	pkg of 20 μg
浓度	≥0.2 mg/mL
杂质	<0.01 EU/μg endotoxin, tested
颜色	clear
UniProt登记号	P50592
运输	dry ice
储存温度	?70℃
Gene Information	mouse ... Tnfsf10(22035)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable