TRAIL, Soluble human

半岛bd体育手机客户端 说明

一般描述

TNF-related apoptosis-inducing ligand (TRAIL; Apo2L; CD253; TNFSF10) is a type II transmembrane protein of about 34kDa. Like most members of the tumor necrosis factor (TNF) superfamily of cytokines, TRAIL can be cleaved at the cell surface by metalloproteases to form a soluble molecule. The gene encoding TRAIL is localized on human chromosome 3q26.31.

应用

TRAIL, soluble human has been used in cell culture, chemical transfection and in caspase-3/7 activity assay.

生化/生理作用

Trimerized TNF-related apoptosis-inducing ligand (TRAIL) triggers apoptosis upon ligation of cell surface TRAIL-receptor 1 (R1) and/or TRAIL-R2 by inducing the formation of the so-called multiprotein death-inducing signaling complex (DISC). Active TRAIL forms trimers and specifically binds to five distinct receptors: TRAIL-receptors 1,-2,-3,-4 {TRAIL-R1 (DR4; Apo2; CD261; TNFRSF10A), TRAIL-R2 (DR5; KILLER; TRICK2A; TRICK2B; CD262; TNFRSF10B), TRAIL-R3 (DcR1; LIT; TRID; CD263; TNFRSF10C), TRAIL-R4 (DcR2; TRUNDD; CD264; TNFRSF10D)} and osteoprotegerin (OPG; OCIF; TNFRSF11B).

外形

Solution in 20 mM HEPES, pH 7.9, 120 mM NaCl, 0.01% Tween 20, 1% sucrose, 1 mM DTT.

其他说明

The extracellular domain of human TRAIL (aa 95-281) is fused at the N-terminus to a His-tag and a linker peptide. The active multimeric conformation is stabilized by an inserted mutation allowing an additional CC-bridge.

半岛bd体育手机客户端 性质

生物来源	human
重组	expressed inE. coli
标签	His tagged
测定	>98% (SDS-PAGE)
形式	liquid
分子量	~26 kDa
包装	pkg of 20 μg
浓度	≥0.2 mg/mL
technique(s)	activity assay: suitable cell culture | mammalian: suitable transfection: suitable
杂质	<0.01 EU/μg endotoxin, tested
颜色	clear
UniProt登记号	P50591
运输	dry ice
储存温度	?70℃
Gene Information	human ... TNFSF10(8743)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable