半岛bd体育手机客户端 说明
应用
Useful for kinase assay and Western blot.
生化/生理作用
Recombinant human tag-free Tau-441 was co-expressed with TTBK1 inE. colicells. The Tau-441 was phosphorylated by TTBK1 in vivo andin vitroprior to the final chromatography purification. Tau-441 or Tau-F is a member of the Tau family of proteins which function to stabilize the microtubules by binding to them. Tau proteins are subject to phosphorylation and this phenomenon regulates the association of the Tau protein with the microtubules(1). Deposits of Alzheimer′s disease AD-associated proteins, such as hyperphosphorylated Tau, as well as other shared misfolded proteins, such as, β-amyloid precursor protein (βAPP), ubiquitin, and various chaperones and protein kinases are thought to play a pathologic role in the cognitive decline and muscular failure. Malfunctioning of Tau proteins is associated with microtubules disintegration and collapsing of the neuronal transport system(2).
重悬
Formulated in 50mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.
储存及稳定性
Store in aliquots at -70℃. Avoid freeze/thaw cycles.
半岛bd体育手机客户端 性质
| 重组 | expressed inE. coli |
| 测定 | 70-95% (SDS-PAGE) |
| 形式 | frozen liquid |
| 分子量 | ~64-70 kDa |
| 浓度 | 0.2 μg/μL |
| 登记号 | P10636-8 |
| 运输 | dry ice |
| 储存温度 | ?70℃ |
| Gene Information | human ... MAPT(4137) |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
