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T4 Beta-glucosyltransferase

品牌
Sigma-Aldrich
货号
SRP0416
规格纯度
recombinant, expressed inE. coli, ≥83% (SDS-PAGE)
价格
6998.47 *本价格含增值税费
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半岛bd体育手机客户端 名称:
T4 Beta-glucosyltransferase
hydroxymethyl cytosine
UDP glucose-DNA ?-glucosyltransferase
T4-phage beta-glucosyltransferase
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

T4-phage β?glucosyltransferase, also known as UDP glucose-DNA β-glucosyltransferase, (Genbank Accession No. NP_049658) amino acids 1-351(end) with C-terminal His-tag, MW= 41.6 kDa, expressed inEscherichia coli.

应用

Useful for the differentiation of hydroxymethylcytosine (HMC) from methylcytosine in DNA, via glucosylating HMC and protecting HMC from endonuclease cleavage.

生化/生理作用

T4-phage β-glucosyltransferase is involved in the transfer of glucose from uridine diphosphoglucose (UDP-Glc) to 5-hydroxymethylcytosine (5-HMC) in double-stranded DNA of the T4-phage. Ions such as Mg2+, Mn2+and Ca2+activate this enzyme.

外形

Formulated in 200 mM imidazole and 20% glycerol.

基本信息

NACRES NA.32

半岛bd体育手机客户端 性质

生物来源 Escherichia coli
重组 expressed inE. coli
测定 ≥83% (SDS-PAGE)
形式 aqueous solution
分子量 41.6 kDa
包装 pkg of 100 μg
NCBI登记号 NP_049658
UniProt登记号 P04547
运输 dry ice
储存温度 ?70℃
Gene Information bacteriophage T4 ... B-GT(1258765)

安全信息

象形图 GHS07GHS08
警示用语: Danger
危险声明 H315 - H319 - H360D
预防措施声明 P202 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313
危险分类 Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
储存分类代码 6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
WGK WGK 1
闪点(F) Not applicable
闪点(C) Not applicable

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