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Sphingosine kinase 2 Active from mouse

品牌
Sigma-Aldrich
货号
SRP0286
规格纯度
recombinant, expressed in baculovirus, ≥70% (SDS-PAGE)
价格
8417.51 *本价格含增值税费
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数量
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半岛bd体育手机客户端 名称:
Sphingosine kinase 2 Active from mouse
SK2
SPK 2
SPHK2
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Mouse Sphingosine kinase 2 ( GenBank Accession No. NM_203280), full length with N-terminal His tag, MW = 68 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

应用

Sphingosine kinase 2 is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Sphingosine kinase 2 has been used in a study to assess blood–brain barrier protection in hypoxic preconditioning-induced cerebral ischemic tolerance. Sphingosine kinase 2 has also been used in a study to investigate sensitization of human colon cancer cells to sodium butyrate-induced apoptosis.

单位定义

One unit of sphingosine kinase activity is defined as the amount of enzyme required to produce 1 pmol of S1P/min

外形

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.

制备说明

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70℃. Note: Enzyme is very sensitive to freeze/thaw cycles.

基本信息

NACRES NA.54

半岛bd体育手机客户端 性质

重组 expressed in baculovirus
测定 ≥70% (SDS-PAGE)
形式 aqueous solution
specific activity ≥2 units/μg
分子量 68 kDa
NCBI登记号 NM_203280
运输 dry ice
储存温度 ?70℃
Gene Information mouse ... SPHK2(56848)

Sigma-Aldrich

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