半岛bd体育手机客户端 说明
一般描述
Mouse Sphingosine kinase 1 (GenBank Accession No. AF415213), full length with N-terminal His tag, MW = 47.1 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
应用
Sphingosine kinase 1a is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Sphingosine kinase 1a has been used in a study to assess the design of sphingosine kinase inhibitors to promote apoptosis of chemotherapeutic resistant cancer cells. Sphingosine kinase 1a has also been used in a study to investigate novel biomarkers for early prostate cancer detection.
单位定义
One unit of sphingosine kinase activity is defined as the amount of enzyme required to produce 1 pmol of S1P/min
外形
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.
制备说明
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70℃. Note: Enzyme is very sensitive to freeze/thaw cycles.
基本信息
| NACRES | NA.54 |
半岛bd体育手机客户端 性质
| 重组 | expressed in baculovirus |
| 测定 | ≥70% (SDS-PAGE) |
| 形式 | aqueous solution |
| specific activity | ≥20 units/μg |
| 分子量 | 47.1 kDa |
| NCBI登记号 | AF415213 |
| 运输 | dry ice |
| 储存温度 | ?70℃ |
| Gene Information | mouse ... SPHK1(8877) |
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