SMURF1 Active human

半岛bd体育手机客户端 说明

一般描述

Human SMURF 1 (GenBank Accession No. NM_020429), amino acids 150-end, with N-terminal DDDDK tag (FLAG) MW = 70 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

应用

Useful in conjunction with E1 and E2 for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

单位定义

One unit is defined as the amount of enzyme required to transfer 1 pmol of ubiquitin to substrate/min at 37℃.

外形

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 100 μg/ml FLAG peptide, 20% glycerol and 3 mM DTT.

制备说明

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70℃. Note: Enzyme is very sensitive to freeze/thaw cycles.

基本信息

NACRES

NA.32

半岛bd体育手机客户端 性质

生物来源	human
重组	expressed in baculovirus infected insect cells
测定	≥80% (SDS-PAGE)
形式	aqueous solution
specific activity	≥0.0055 pmol/min-μg
分子量	70 kDa
包装	pkg of 20 μg
储存条件	avoid repeated freeze/thaw cycles
浓度	>0.02 mg/mL
NCBI登记号	NM_020429
UniProt登记号	Q9HCE7
运输	dry ice
储存温度	?70℃
Gene Information	human ... SMURF1(57154)