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Sirtuin 2 human

品牌
Sigma-Aldrich
货号
SRP0116
规格纯度
recombinant, expressed inE. coli, ≥80% (SDS-PAGE)
价格
8365.25 *本价格含增值税费
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数量
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半岛bd体育手机客户端 名称:
Sirtuin 2 human
SIR2L2
SIRT2
sir2-like 2
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Silent information regulator 2 (Sir2) or sirtuin 2 is encoded by the gene mapped to human chromosome 19q13.2. It belongs to class Ib of sirtuin protein family. Sir2 is characterized with a catalytic domain containing two different domains that bind NAD and the acetyl-lysine substrate, respectively. Apart from this, it also constitutes variable NH2 and COOH-terminal domains involved in the regulation of subcellular localizations and catalytic activity. sirtuin 2 is mainly expressed in cytosol but its migration between cytosol and nucleus facilitates the deacetylation of both α-tubulin and histones.
Human Sirtuin 2, GenBank Accession No. NM_030593, amino acids 13-319 with C-terminal His tag, MW = 35.5kDa, expressed inEscherichia coliexpression system.

应用

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

生化/生理作用

Silent information regulator 2 (Sir2) acts as a nicotine adenine dinucleotide (NAD) dependent deacetylase and is implicated in various biological processes such as regulation of transcriptional repression, recombination, the cell-division cycle, microtubule organization, and cellular responses to DNA-damaging agents. In addition, sirtuins also regulate the process involved in aging. Sir2 plays a vital role in mammalian metabolic homeostasis and is considered to be a potent therapeutic target for the treatment of type 2 diabetes. Sir2 has a crucial role in reduction of microglial activation and brain inflammation. Therefore, loss of Sir2 activity increases the risk of susceptibility to neurodegenerative diseases.

单位定义

One unit is defined as the amount of enzyme required to deacetylate 1 pmol of substrate/min at 37℃.

外形

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.

制备说明

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70℃. Note: Enzyme is very sensitive to freeze/thaw cycles.

基本信息

NACRES NA.32

半岛bd体育手机客户端 性质

生物来源 human
重组 expressed inE. coli
测定 ≥80% (SDS-PAGE)
形式 aqueous solution
分子量 35.5 kDa
包装 pkg of 100 μg
储存条件 avoid repeated freeze/thaw cycles
浓度 >0.02 mg/mL
NCBI登记号 NM_030593
UniProt登记号 Q8IXJ6
运输 dry ice
储存温度 ?70℃
Gene Information human ... SIRT2(22933)

安全信息

储存分类代码 10 - Combustible liquids
WGK WGK 1
闪点(F) Not applicable
闪点(C) Not applicable

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