半岛bd体育手机客户端 说明
一般描述
Human Sirtuin 1, GenBank Accession No. NM_012238), amino acids 193-741 with N-terminal ST tag, MW = 87.2 kDa, expressed inE. coliexpression system.
应用
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
单位定义
One unit is defined as the amount of enzyme required to deacetylate 1 pmol of substrate/min at 37℃.
外形
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20 and 20% glycerol.
制备说明
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70℃. Note: Enzyme is very sensitive to freeze/thaw cycles.
基本信息
NACRES | NA.32 |
半岛bd体育手机客户端 性质
生物来源 | human |
重组 | expressed inE. coli |
测定 | ≥60% (SDS-PAGE) |
形式 | aqueous solution |
分子量 | 87.2 kDa |
包装 | pkg of 100 μg |
储存条件 | avoid repeated freeze/thaw cycles |
NCBI登记号 | NM_012238 |
UniProt登记号 | Q96EB6 |
运输 | dry ice |
储存温度 | ?70℃ |
Gene Information | human ... SIRT1(23411) |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich