RNA Polymerase II, p33 subunit human

半岛bd体育手机客户端 说明

生化/生理作用

hRPB3 (p33) is a highly conserved subunit shared by all three RNA polymerases. It has been shown to be in close contact to promoter DNA when pol II is recruited into the preinitiation complex. RPB3 has also been implicated in direct protein-protein contacts with transcription factor IIB, Rap30 subunit of transcription factor IIF, and gene-specific modulator proteins, such as the hepatitis B virus transactivator protein X or an inhibitor of pol II, RMP (RPB3-mediating protein). Therefore, RPB3 is facilitating the communication between the pol II core and a variety of basal and gene-specific transcription factors. In the pol II complex, RPB3 interacts with RPB5 and the RPB3–RPB5 interaction is intensified in the presence of three subunits, RPB7, RPB8 and RPB11. RPB3 also makes direct contacts with both RPB1 and RPB2, the two large subunits of the polymerase complex.

外形

Clear and colorless frozen liquid solution

制备说明

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

基本信息

NACRES

NA.26

半岛bd体育手机客户端 性质

生物来源	human
重组	expressed inE. coli
测定	≥80% (SDS-PAGE)
形式	frozen liquid
分子量	~33.5 kDa
包装	pkg of 10 μg
储存条件	avoid repeated freeze/thaw cycles
浓度	350 μg/mL
technique(s)	western blot: suitable
颜色	clear colorless
NCBI登记号	NM_032940
UniProt登记号	P19387
运输	dry ice
储存温度	?70℃
Gene Information	human ... POLR2C(5432)

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable