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Recombinant 2019-nCoV Spike S1 Protein with His tag

品牌
Sigma-Aldrich
货号
SAB5700594
包装型号
规格纯度
价格
5135.53 *本价格含增值税费
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半岛bd体育手机客户端 名称:
Recombinant 2019-nCoV Spike S1 Protein with His tag
S glycoprotein Subunit1
S1 protein
novel coronavirus s1 Protein
Spike glycoprotein Subunit1
Spike protein S1
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates thisinteraction.The S protein plays key parts in the induction of neutralizing-antibody and T-cellresponses, as well as protective immunity.
Recombinant SARS-COV-2(2019-nCoV) Spike S1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Val16-Arg685) of SARS-COV-2(2019-nCoV) Spike S1 (Accession #YP_009724390.1) fused with a 6XHis tag at the C-terminus.

生化/生理作用

Measured by its binding ability in a functional ELISA. Immobilized Recombinant SARS-COV-2 Spike S1 at 2μg/mL (100 μL/well) can bind Recombinant Human ACE2 with a linear range of 0.5-8.7 ng/mL.|Immobilized Human ACE2 on COOH Chip, can bind SARS-COV-2 Spike S1 with an affinity constant of 11.4 nM as determined in a SPR assay.

重悬

For lyophilized protein : Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.

储存及稳定性

Store the lyophilized protein at -20℃ to -80℃ for long term. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8 ℃ for up to 1 week. This product is stable at ≤ -70℃ for up to 6 months from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses,in vitrodiagnostic uses,ex vivoorin vivotherapeutic uses or any type of consumption or application to humans or animals.

Data presented is the available current product information and provided as-is. These products have not been tested or verified in any additional applications, sample types, including any clinical use. Experimental conditions must be empirically derived by the user. Our Antibody Guarantee only covers tested applications stated herein and conditions presented in our product information and is not extended to publications.

半岛bd体育手机客户端 性质

重组 expressed in HEK 293 cells
质量水平 100
测定 >90% (SDS-PAGE)
>95% (HPLC)
形式 lyophilized powder
分子量 75.8 kDa
杂质 <1.0 EU/μg protein (LAL test)
UniProt登记号 P0DTC2
运输 wet ice
储存温度 ?20℃
Gene Information SARS virus ... S(43740568)

安全信息

储存分类代码 11 - Combustible Solids
WGK WGK 3
闪点(F) Not applicable
闪点(C) Not applicable

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